细胞不长了，请问怎么办？ - 未名空间MITBBS历史存档

国际科技财经博客移民网络热点娱乐民生时事公众号

Redian新闻

>未名空间

>Biology - 生物学

细胞不长了，请问怎么办？

细胞不长了，请问怎么办？# Biology - 生物学

e*e2012-06-05 07:06

1 楼

tranform recombinant plasmid in BL21(DE3)pLysS Competent Cells, observed
colonies in Petri dish culture, 10 mL cuture was good. cells stopped growing
when we put them into 1 liter LB broth. 细胞不长了，请问怎么办？

i*l2012-06-05 07:06

2 楼

determine pH of your 1 liter LB
spin down 10ml ecoli, resuspend with fresh media before transfer
lack of O2? there is special flask for large scale biomass culture
colony contaminated and outgrown in the 10ml tube?

growing

【在 e******e 的大作中提到】

: tranform recombinant plasmid in BL21(DE3)pLysS Competent Cells, observed
: colonies in Petri dish culture, 10 mL cuture was good. cells stopped growing
: when we put them into 1 liter LB broth. 细胞不长了，请问怎么办？

e*e2012-06-05 07:06

3 楼

谢谢。请给一个买special flask的 link.

i*l2012-06-05 07:06

4 楼

http://www.krackeler.com/products/1157-Erlenmeyer/15140-Corning

【在 e******e 的大作中提到】

: 谢谢。请给一个买special flask的 link.