d*i
2 楼
google了半天拿不定主意。。有商用的最好。
s*3
3 楼
Do you still have a valid coupon?
【在 b****r 的大作中提到】
: 用了coupon和gift card后$145就买下了。
【在 b****r 的大作中提到】
: 用了coupon和gift card后$145就买下了。
a*e
4 楼
SV40 NLS: PKKKRKV
l*l
5 楼
求細節。
d*i
6 楼
我是想把目的蛋白和核定位信号序列融合构成plasmid，希望转染后表达的蛋白都进到
细胞核内。这种融合的时候在NLS和目的基因之间要加其他序列吗？不会就这么硬生生
的连在一起吧？谢谢!
细胞核内。这种融合的时候在NLS和目的基因之间要加其他序列吗？不会就这么硬生生
的连在一起吧？谢谢!
b*y
7 楼
Check Yuh Min Chook's studies. There are many options. Of course, the one
suggested by ahche works. As far as linkers are concerned, a GGS linker in
between should do it. If your protein is intrinsically disordered at the
location of linkage, direct fusion should also do it. What matters is the
availability of the NLS to importin(s).
suggested by ahche works. As far as linkers are concerned, a GGS linker in
between should do it. If your protein is intrinsically disordered at the
location of linkage, direct fusion should also do it. What matters is the
availability of the NLS to importin(s).
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