f*t
2 楼
女人YY起来,古今中外都是一样。电影里是女主,微微一笑里是编剧。好可怕。
e*r
3 楼
试过restore,但是出现3194错误。是因为不开放SHSH的原因?
P*y
4 楼
Does anyone know currently whether it is feasible to do whole-genome
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
N*n
6 楼
some people did microarray analysis using LCM tissue. it could be doable.
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
P*y
7 楼
We have no problem to do microarray and RNA-seq with LCM tissue. There are
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
P*y
8 楼
Does anyone know currently whether it is feasible to do whole-genome
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
N*n
9 楼
some people did microarray analysis using LCM tissue. it could be doable.
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
P*y
10 楼
We have no problem to do microarray and RNA-seq with LCM tissue. There are
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
s*9
11 楼
DNA should be easier because you do not need to worry about RNA degradation
during the staining and capture process.
It is a reference for classic sequence of DNA sample using LCM tissues.
Good luck.
PMID:19934021
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
during the staining and capture process.
It is a reference for classic sequence of DNA sample using LCM tissues.
Good luck.
PMID:19934021
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
P*y
12 楼
Thank you.
However what I want is a protol that can amplify the whole genomic DNA from
LCM tissue, not several target genes. The point here is "whole-genome"
bisulphite sequencing.
degradation
【在 s****9 的大作中提到】
: DNA should be easier because you do not need to worry about RNA degradation
: during the staining and capture process.
: It is a reference for classic sequence of DNA sample using LCM tissues.
: Good luck.
: PMID:19934021
However what I want is a protol that can amplify the whole genomic DNA from
LCM tissue, not several target genes. The point here is "whole-genome"
bisulphite sequencing.
degradation
【在 s****9 的大作中提到】
: DNA should be easier because you do not need to worry about RNA degradation
: during the staining and capture process.
: It is a reference for classic sequence of DNA sample using LCM tissues.
: Good luck.
: PMID:19934021
b*n
13 楼
Why do you need to amplify genome DNA? QIAGEN just had a kit to make BS
library with 10ng of genome DNA.
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
library with 10ng of genome DNA.
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
P*y
15 楼
There should be a number of published papers describing the protocol. You
can google it.
Here are a couple that I found:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156186/
http://dev.biologists.org/content/140/2/423.full
【在 s******r 的大作中提到】
: 能不能分享一下LCM tissue做microarray和RNA-seq的protocol?
can google it.
Here are a couple that I found:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156186/
http://dev.biologists.org/content/140/2/423.full
【在 s******r 的大作中提到】
: 能不能分享一下LCM tissue做microarray和RNA-seq的protocol?
相关阅读
PERT assay请教从mouse tissue里提蛋白做Westernguava flow cytometry来自国内关于生物博后的正能量!请问研究方向哪个比较好?求助:哪位朋友有向国内寄送病原菌菌种的经验求评价boss的模板生命啊,我好害怕求一片JCI的paperDNA-damage rH2AX ChIP-qPCR 正对照的选取.哪儿有提供筛compound对肿瘤细胞活性的公司?谢谢大家有没有好的 protein topology 作图软件推荐?求助!大家养过human primary endothelial cells吗?包子求文献一篇跪求审稿机会Flu virus 细胞粘附一问?上海神经所胡海岚研究组再发Science文章这个是不是有点过分Paper Help, Please.做中和抗体的牛人帮个忙