Redian新闻
>
48/96孔板上养的人胚胎干细胞有没有简易冷冻的方法?
avatar
48/96孔板上养的人胚胎干细胞有没有简易冷冻的方法?# Biology - 生物学
l*y
1
我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信的模板,希望对大家有帮助:
Dear Dr. XXX,
My name is XXX and I am currently working as a XXX in XXX. My research mainly focuses on XXX.(Please refer to the attached resume for more details of my research and publications).
In last six years, I have reviewed several manuscripts for XXX with some experiences on
peer review. I find that manuscript reviewing is ver
avatar
a*n
2
老鼠干细胞可以trypsinize后中和,然后直接加20%DMSO。可是人干细胞不能
trypsinize啊?
avatar
H*9
3
Thanks for kindly sharing your template.
avatar
i*0
4
为什么人的不可以,你做EB assay的时候或者其他的diff assay的时候,就不要单细胞
悬液了么?我做的是小鼠的胚胎干。但是我觉得你可以用小鼠干细胞冷冻的方法,冻人
的干细胞。不过我我们是用20%的Freezing medium。 其实就是20% DMSO, 20% SERUM,
60% DMEM.
LZ可以小规模试试看,然后告诉大家结果呀。
avatar
c*g
5
信写得不错
你这邮件发得也够狠的啊 : )

荐的,在
6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续
续的一共来
了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信
的模
板,希望对大家有帮助:
mainly focuses on XXX.(Please refer to the attached resume for more
details of my research and publications).
some experiences on
career future in
taking responsibilities
application. Therefore,
convenience in my research fields/interests, including but not limited
to XXX.
receiving your

【在 l********y 的大作中提到】
: 我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信的模板,希望对大家有帮助:
: Dear Dr. XXX,
: My name is XXX and I am currently working as a XXX in XXX. My research mainly focuses on XXX.(Please refer to the attached resume for more details of my research and publications).
: In last six years, I have reviewed several manuscripts for XXX with some experiences on
: peer review. I find that manuscript reviewing is ver

avatar
a*n
6
因为人胚胎干细胞解离成单细胞后会分化或者死亡,所以一般细胞传代时是不用
trypsin的。这时人和鼠干细胞不同的地方。
avatar
y*p
7
我来版上没几天,最先记住的ID就是楼主和cleverpig.
狂赞两个热心人。
avatar
i*0
8
我明天问问我们实验室做过人的ips的,看他怎么说。
avatar
t*7
9
严重同意,动辄100-200封啊!
我说我咋老凑不够呢,还是修行不到啊!继续发!!

【在 c*******g 的大作中提到】
: 信写得不错
: 你这邮件发得也够狠的啊 : )
:
: 荐的,在
: 6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续
: 续的一共来
: 了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信
: 的模
: 板,希望对大家有帮助:
: mainly focuses on XXX.(Please refer to the attached resume for more

avatar
m*z
10
可以单细胞,recover的时候加rock inhibitor就行了。

【在 a******n 的大作中提到】
: 因为人胚胎干细胞解离成单细胞后会分化或者死亡,所以一般细胞传代时是不用
: trypsin的。这时人和鼠干细胞不同的地方。

avatar
d*n
11
谢谢。
avatar
s*r
12
以96孔板为例,吸掉培养基,加200-250ul冻存medium,然后用排枪在孔里搅合搅合,
把克隆刮下来就行了。
avatar
l*y
13
i copied this letter from the website and made some minor change.
we sent 10-20 E-mails last summer but did not get any reviews, so ~100 is
the right number to get some reviews.
good luck everyone!!
avatar
a*n
14
多谢热心帮忙,等你的回音。

★ 发自iPhone App: ChineseWeb - 中文网站浏览器

【在 i*********0 的大作中提到】
: 我明天问问我们实验室做过人的ips的,看他怎么说。
avatar
d*w
15
good to learn, thanks!
avatar
a*n
16
有经验的来了!复活的程序是怎么样的?要离心吗?

★ 发自iPhone App: ChineseWeb - 中文网站浏览器

【在 s********r 的大作中提到】
: 以96孔板为例,吸掉培养基,加200-250ul冻存medium,然后用排枪在孔里搅合搅合,
: 把克隆刮下来就行了。

avatar
s*7
17
good to know! Thanks

【在 l********y 的大作中提到】
: i copied this letter from the website and made some minor change.
: we sent 10-20 E-mails last summer but did not get any reviews, so ~100 is
: the right number to get some reviews.
: good luck everyone!!

avatar
i*0
18
他们都是在48孔做,基本上是dispase去消化一下,然后吹打。
最好不要做单细胞悬液。
可哟考虑用rock inhibitor,但是听说会增加mutation的几率。

【在 a******n 的大作中提到】
: 多谢热心帮忙,等你的回音。
:
: ★ 发自iPhone App: ChineseWeb - 中文网站浏览器

avatar
j*a
19
mark 一下,明天我就开始找争取100封!

荐的,在
6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续
续的一共来
了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信
的模
板,希望对大家有帮助:
mainly focuses on XXX.(Please refer to the attached resume for more
details of my research and publications).
some experiences on
career future in
taking responsibilities
application. Therefore,
convenience in my research fields/interests, including but not limited
to XXX.
receiving your

【在 l********y 的大作中提到】
: 我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信的模板,希望对大家有帮助:
: Dear Dr. XXX,
: My name is XXX and I am currently working as a XXX in XXX. My research mainly focuses on XXX.(Please refer to the attached resume for more details of my research and publications).
: In last six years, I have reviewed several manuscripts for XXX with some experiences on
: peer review. I find that manuscript reviewing is ver

avatar
a*n
20
dispase之后什么步骤,直接加freezing media吗?不需要洗去或中和dispase?

【在 i*********0 的大作中提到】
: 他们都是在48孔做,基本上是dispase去消化一下,然后吹打。
: 最好不要做单细胞悬液。
: 可哟考虑用rock inhibitor,但是听说会增加mutation的几率。

avatar
b*y
21
谢谢楼主!大好人呐。顺便请教一下
你们专业领域有那么多杂志可发吗?100多?
就算我想把工作做到家,可是我找不到那么多适合我的方向的杂志。这可咋办啊?郁闷。

荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期
间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member
,分享一下我们发信的模板,希望对大家有帮助:
mainly focuses on XXX.(Please refer to the attached resume for more details
of my research and publications).
experiences on
career future in
responsibilities
application. Therefore,
convenience in my research fields/interests, including but not limited to
XXX.
receiving your

【在 l********y 的大作中提到】
: 我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信的模板,希望对大家有帮助:
: Dear Dr. XXX,
: My name is XXX and I am currently working as a XXX in XXX. My research mainly focuses on XXX.(Please refer to the attached resume for more details of my research and publications).
: In last six years, I have reviewed several manuscripts for XXX with some experiences on
: peer review. I find that manuscript reviewing is ver

avatar
l*y
22
我们大概发了60-70个杂志吧,刚开始没有经验,看到有e-mail address就发,同一
个杂志发了都有7-8封,后来有编辑回信了,说每个人都收到邮件了,发的太多了,他
们内部会转发这种邮件的。教训就是一个杂志发一封邮件就行了。
60-70个杂志应该问题不大吧,不管什么专业。

闷。
member
details

【在 b********y 的大作中提到】
: 谢谢楼主!大好人呐。顺便请教一下
: 你们专业领域有那么多杂志可发吗?100多?
: 就算我想把工作做到家,可是我找不到那么多适合我的方向的杂志。这可咋办啊?郁闷。
:
: 荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期
: 间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member
: ,分享一下我们发信的模板,希望对大家有帮助:
: mainly focuses on XXX.(Please refer to the attached resume for more details
: of my research and publications).
: experiences on

avatar
I*t
23
多谢!
avatar
w*e
24
mark!!!
avatar
b*g
25
thanks much. very helpful.
avatar
a*u
26
thanks a lot.
avatar
s*0
27
Thanks
avatar
h*i
28
thanks
avatar
q*2
29
发信人: lovelypony (lovelypony), 信区: Immigration
标 题: 攒人品-要review的模板
发信站: BBS 未名空间站 (Tue Sep 21 23:05:43 2010, 美东)
我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐
的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间
陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,
分享一下我们发信的模板,希望对大家有帮助:
Dear Dr. XXX,
My name is XXX and I am currently working as a XXX in XXX. My research
mainly focuses on XXX.(Please refer to the attached resume for more details
of my research and publications).
In last six years, I have reviewed several manuscripts for XXX with some
experiences on
peer review. I find that manuscript reviewing is very helpful to my career
future in
terms of improving professional expertise, broadening my horizons, taking
responsibilities
in scientific communities, and helping me with permanent residency
application. Therefore,
I am very interested in reviewing manuscripts for XXX at your earliest
convenience in my research fields/interests, including but not limited to
XXX.
Thank you very much for your consideration and I am looking forward to
receiving your
reply soon!
Best regards,
xxx
avatar
e*p
30
请问,这样的request email要发给谁呢?杂志的associate editors?还是editor?
怎么才能找到他们的联系方式呢?谢谢啦!

荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期
间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member
,分享一下我们发信的模板,希望对大家有帮助:
mainly focuses on XXX.(Please refer to the attached resume for more details
of my research and publications).
experiences on
career future in
responsibilities
application. Therefore,
convenience in my research fields/interests, including but not limited to
XXX.
receiving your

【在 l********y 的大作中提到】
: 我们在大量发邮件要review之前大概审过6-7篇稿子,都是老板或者collaborator推荐的,在6月份的时候发了大概100-120封邮件,第一周内有2-3个稿子,后来2个月期间陆陆续续的一共来了大概14-15篇审稿,和一个没分杂志的editorial board member,分享一下我们发信的模板,希望对大家有帮助:
: Dear Dr. XXX,
: My name is XXX and I am currently working as a XXX in XXX. My research mainly focuses on XXX.(Please refer to the attached resume for more details of my research and publications).
: In last six years, I have reviewed several manuscripts for XXX with some experiences on
: peer review. I find that manuscript reviewing is ver

avatar
l*n
31
Thanks
相关阅读
logo
联系我们隐私协议©2024 redian.news
Redian新闻
Redian.news刊载任何文章,不代表同意其说法或描述,仅为提供更多信息,也不构成任何建议。文章信息的合法性及真实性由其作者负责,与Redian.news及其运营公司无关。欢迎投稿,如发现稿件侵权,或作者不愿在本网发表文章,请版权拥有者通知本网处理。