我有个朋友也是做HBV的,他的说法和你说的有些相似: 1. 很多group都repeat了李文辉的发现,NTCP overexpresed HepG2 cells can be infected by HBV in vitro.很多组都在用了。 2. 李文辉已经拿到了NTCP transgenic老鼠,但是老鼠不能被感染。后面的东西还很 complicated。是receptor还是co-receptor还不清楚。
you guy if Li and or Sui lab could get 清晰的条带 for it by any modified protocol, the manuscript at least will be released on Cell Host&Microbe, http://www.bioxbio.com/if/html/CELL-HOST-MICROBE.html or if got new mechanism of HBV Immuo aspect then to N Immu, http://www.bioxbio.com/if/html/NAT-IMMUNOL.html Do you think those labs only think [email protected] is better than Cell Host&Microbe similar rank other journals. ps: But key point is 让人怀疑作者检测到的human HBV infected cccDNA是否是真的 human HBV infected cccDNA? details please refer . 文章中用Southern blotting检测到了cccDNA的形成。cccDNA是HBV转录的膜板,被认 为是HBV持续感染的关键因素。从结果来看cccDNA的形成时间与HBV蛋白、RNA和DNA相比 比较早,尤其量很高。其实对于HBV来说哪怕是CMV启动的HBV(比如pCMV-HBV)cccDNA 的量都是很低的,更不要说是自身启动子启动的了。在NTCP介导的HBV复制水平整体很 低的情况下,具有高水平的cccDNA形成,让人怀疑作者检测到的cccDNA是否是真的 cccDNA,所以作者必须证明他们所指示的条带是真的是cccDNA,必须用EcoRI酶切( cccDNA能被EcoRI线性化)和加热煮沸cccDNA(沸腾后不会被解成单链)来确证,同时 必须要有阳性对照。当然对于鸭乙肝病毒(DHBV) http://blog.sciencenet.cn/blog-279293-633906.html http://www.mitbbs.com/article_t1/Biology/31752161_0_3.html 58th floor
【在 M******n 的大作中提到】 : 最新进展: : http://jvi.asm.org/content/early/2014/01/02/JVI.03478-13 : Viral entry of Hepatitis B and D viruses and bile salts transportation share : common molecular determinants on sodium taurocholate cotransporting : polypeptide