c*r
2 楼
除了工资里扣的和公司要MATCH的,还该不该买,将来真的能用上吗?
s*y
3 楼
最近在做酵母实验,突然想起来这个问题:酵母用600nm的波长测的时候,正好是
10millian cell = 1.0 OD.
这个有什么来源讲究么?是不是当初定义OD的时候是用大颗粒的数目来定义的?
10millian cell = 1.0 OD.
这个有什么来源讲究么?是不是当初定义OD的时候是用大颗粒的数目来定义的?
l*s
6 楼
no, pending on your yeast strain.
c*r
8 楼
觉得这钱交进去了,如同肉包子打狗,将来自己能不能拿回来也不一定。退休后还得一
点一点拿,不能一次拿,跟买人寿保险差不多,名义上省税,到时都让政府给收了。
点一点拿,不能一次拿,跟买人寿保险差不多,名义上省税,到时都让政府给收了。
s*e
11 楼
纯属巧合。OD是用数学方法定义的(入射出射光比值的对数),不像米摄氏度那样使用
物理方法定义。多数透光溶液通过调整浓度来扫描分光光度谱总是可以找到一个等于一
的波长,只不过你的质点正好在一个整数波长下对应了一个整数的吸光度数值。
物理方法定义。多数透光溶液通过调整浓度来扫描分光光度谱总是可以找到一个等于一
的波长,只不过你的质点正好在一个整数波长下对应了一个整数的吸光度数值。
c*o
14 楼
简单,钱够多就投,钱不够就不投
s*y
15 楼
谢谢大家的解答!这么看来是一个巧合。
z*3
17 楼
OD600 doesn’t give absolute cell concentration
OD600 is cell dependent
Need to independently measure cell concentration so that the two can be
related.
Measure absolute cell concentration by dilution and plating.
Plating measures cfu
Standard curve = plot OD600 vs. cfu
【在 s******y 的大作中提到】
: 最近在做酵母实验,突然想起来这个问题:酵母用600nm的波长测的时候,正好是
: 10millian cell = 1.0 OD.
: 这个有什么来源讲究么?是不是当初定义OD的时候是用大颗粒的数目来定义的?
OD600 is cell dependent
Need to independently measure cell concentration so that the two can be
related.
Measure absolute cell concentration by dilution and plating.
Plating measures cfu
Standard curve = plot OD600 vs. cfu
【在 s******y 的大作中提到】
: 最近在做酵母实验,突然想起来这个问题:酵母用600nm的波长测的时候,正好是
: 10millian cell = 1.0 OD.
: 这个有什么来源讲究么?是不是当初定义OD的时候是用大颗粒的数目来定义的?
c*r
18 楼
现在就纠结这事,如果不买,税拿不回来,如果买多交一笔,税也只省一部分,出去的
更多,拿回来还是以后的事,谁知道以后的事呢?总觉得这事就是个套,钻不钻都为难
。
更多,拿回来还是以后的事,谁知道以后的事呢?总觉得这事就是个套,钻不钻都为难
。
i*a
21 楼
有钱人 also need to diversity their money/investment and lower their taxes.
I don't know how it would work out for the rich but their accountants
maybe doing lots of 401k, roth etc for them to reduce their taxes.
【在 z*********n 的大作中提到】
: 我是说比例,对于有钱人让他们每年Max out 一万五的401k
: 就和每年让你Max out100块一个什么基金一样无聊,说不定一毛钱
: 对于你,每年一万五很痛苦纠结
: 对于有钱人,肯定不纠结,但肯定也懒得去投,无聊
I don't know how it would work out for the rich but their accountants
maybe doing lots of 401k, roth etc for them to reduce their taxes.
【在 z*********n 的大作中提到】
: 我是说比例,对于有钱人让他们每年Max out 一万五的401k
: 就和每年让你Max out100块一个什么基金一样无聊,说不定一毛钱
: 对于你,每年一万五很痛苦纠结
: 对于有钱人,肯定不纠结,但肯定也懒得去投,无聊
z*n
22 楼
that's high mid class, or rich people, might not be richest people, haha
I was just lifting bars
【在 i****a 的大作中提到】
: 有钱人 also need to diversity their money/investment and lower their taxes.
: I don't know how it would work out for the rich but their accountants
: maybe doing lots of 401k, roth etc for them to reduce their taxes.
I was just lifting bars
【在 i****a 的大作中提到】
: 有钱人 also need to diversity their money/investment and lower their taxes.
: I don't know how it would work out for the rich but their accountants
: maybe doing lots of 401k, roth etc for them to reduce their taxes.
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