Hi, I treated groups of transgenic mice with a few chemicals for inhibiting cancer but noticed one chemical may reduce the activity of insulin. A direct consequence should be higher blood glucose level/increased insulin. But the experiment has been completed and there is not much chance to convince my boss to repeat an experiment for measure blood glucose level. I am not in the metabolism field but found that most works in the publications measure the glucose after overnight fasting or at least using fresh blood drops. Can I use the frozen serum (collected for measure cytokines) to measure blood glucose levels? Thanks!
m*h
3 楼
Q~~~
r*g
4 楼
should be ok in theory. But I have never tried frozen serum. Just buy a glucose meter from pharmacy. It works fine in our hands for mice serum.
【在 r***a 的大作中提到】 : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?
h*o
8 楼
serum glucose测试没问题, frozen sample也可以用 但是如果你当时sac 老鼠的时候没有fast, 那么需要很大的N per group 才能检测到 overall的区别 老鼠隔三差五就会去啃一口mouse chow, 所以blood glucose 变化会比较大 除非你的 药像streptozotocin这种强效。
W*7
9 楼
Go ahead give a try. Should be fine. If you do have any problem, we have a machine for measuring glucose level in serum.
【在 r***a 的大作中提到】 : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?
【在 r***a 的大作中提到】 : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?