Nat Biotechnol. 2016 Oct 31. doi: 10.1038/nbt.3701. [Epub ahead of print] Single-cell sequencing of the small-RNA transcriptome. Faridani OR1, Abdullayev I1,2, Hagemann-Jensen M1,3, Schell JP4,5, Lanner F4 ,5, Sandberg R1,2. Author information Abstract Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states. thanks a lot.