S*t
2 楼
故事发生在公元307年,东周时期。虽说在东周这个野蛮闭塞的时期,但是科技和文明
已经赶上现在了。猪脚苏秦是个普通的学生生在普通的家庭,蹲马桶的时候被恶搞的同
学将他的头按进马桶后从此失眠了,1年都没合眼。
搞笑的情节有很多哈哈,一呢苏秦为了睡着天天睡在肌肉男抱枕上(莫非是男同。。妈
妈咪啊),还边听着凤凰传奇的音乐,他的老妈正焦急的时候突然跳起广场舞来了,和
他的邻居们。
后来来了个教授给他用摆钟催眠,摆钟里出来个仙女,仙女说了可以实现苏秦一个愿望
。苏秦立马说请治好我的失眠症吧,仙女的回答是不行,太难了。呵呵呵,真为难那给
我金银财宝吧,不行太简单了。提出的要求通通NG真心瞧不起仙女啊!
后天来,疗养院的李耳登场了,疗养院在距离海平面6ooo千里的海底哎。举个他治愈的
案例吧,对幽闭症患者把他家里的强刷成逼真的天空,待在家里就像呆在大草原的赶脚
,患者想要逃跑结果是不知道哪是门而撞墙上了。
后面就是进入疗养院了,有爱怪装癖的人记不住名字了,裸奔症的孙武武,可以说满足
了口味的人群。。
已经赶上现在了。猪脚苏秦是个普通的学生生在普通的家庭,蹲马桶的时候被恶搞的同
学将他的头按进马桶后从此失眠了,1年都没合眼。
搞笑的情节有很多哈哈,一呢苏秦为了睡着天天睡在肌肉男抱枕上(莫非是男同。。妈
妈咪啊),还边听着凤凰传奇的音乐,他的老妈正焦急的时候突然跳起广场舞来了,和
他的邻居们。
后来来了个教授给他用摆钟催眠,摆钟里出来个仙女,仙女说了可以实现苏秦一个愿望
。苏秦立马说请治好我的失眠症吧,仙女的回答是不行,太难了。呵呵呵,真为难那给
我金银财宝吧,不行太简单了。提出的要求通通NG真心瞧不起仙女啊!
后天来,疗养院的李耳登场了,疗养院在距离海平面6ooo千里的海底哎。举个他治愈的
案例吧,对幽闭症患者把他家里的强刷成逼真的天空,待在家里就像呆在大草原的赶脚
,患者想要逃跑结果是不知道哪是门而撞墙上了。
后面就是进入疗养院了,有爱怪装癖的人记不住名字了,裸奔症的孙武武,可以说满足
了口味的人群。。
f*g
3 楼
iOS早就被cisco 拿走了。当然苹果有钱不怕打官司。还有ipad也是别人早就用的
h*r
4 楼
I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
selection of stable cells. After selection, the selected clonies exhibited
AKT expression through the examintion of HA-tag antibodies in Western blot.
Moreover,the fast tumor formation was observed in nude mice implanted cells
expressing myr-AKT. However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and som
selection of stable cells. After selection, the selected clonies exhibited
AKT expression through the examintion of HA-tag antibodies in Western blot.
Moreover,the fast tumor formation was observed in nude mice implanted cells
expressing myr-AKT. However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and som
s*z
5 楼
斯坦福/伯克利做高分子/生物材料的有名老板有谁啊,
请推荐。
谢谢
请推荐。
谢谢
b*e
6 楼
这有什么奇怪的. 一直都是放限定日期的号, 超过限定时间的不放号啊.
M*e
7 楼
iMovie.com 不知道是谁家的
m*3
8 楼
"However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and some others in
western blot, it is difficult to obtain the signal from western blot"
Do you mean you can not get any signal for all these antibodies? Or there is
no difference between your stable cell line and the parental cell line?
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and some others in
western blot, it is difficult to obtain the signal from western blot"
Do you mean you can not get any signal for all these antibodies? Or there is
no difference between your stable cell line and the parental cell line?
a*g
9 楼
那是 IOS 老乔的 iOS,不一样,哈哈
h*r
10 楼
is
I can not get any signal for all these antibodies. Could you please tell me
the reasons? Thanks!
【在 m**********3 的大作中提到】
: "However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and some others in
: western blot, it is difficult to obtain the signal from western blot"
: Do you mean you can not get any signal for all these antibodies? Or there is
: no difference between your stable cell line and the parental cell line?
m*y
12 楼
maybe the pathway is not activated in your system?
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
m*k
13 楼
是别人剽窃iX的idea。
n*e
14 楼
can you see akt expression?
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
.
cells
Could
【在 h********r 的大作中提到】
: I transferred my HA-tag myr-AKT plasmid into my cells and conducted the
: selection of stable cells. After selection, the selected clonies exhibited
: AKT expression through the examintion of HA-tag antibodies in Western blot.
: Moreover,the fast tumor formation was observed in nude mice implanted cells
: expressing myr-AKT. However, currently, when I used these cells (several
: passages cell culture) for examining phospho-AKT, upstream signals or
: downstream signals such as phospho-GSK,phospho-S6K and som
N*3
17 楼
这种事情别人显然都已经考虑过了。有问题之前也协商好了。
相关阅读
那么多人想转行,就没人研究下美国的高中教师资格证么?孤独求败 求战 五毛党这么没前途的专业?为啥还有人坚持?Paper help, thanks.One paper help!帮忙下两篇文章,谢谢!学化学的下场中山大学生物医学工程系招人 (转载)干燥剂哪里买?无钱付药费遭医院拒收 前诺贝尔化学奖得主离世大米的心动淡泊以明志,宁静以致远 ——屠呦呦团队骨干钟裕蓉印象大家圣诞都去哪里度假?给读化学的师弟师妹一点建议一个工作机会,NJ的Paper please. Thanks.Hawaii开会需要住处的请进化学硕士恳求内推another FREE clinical SAS programming training for pharmaceutical industry复旦赵东元找小三住博士后公寓 (转载)