Re: cross-link# Biology - 生物学
f*p
1 楼
I think Pierce has a very comprehensive guide for protein crosslinking
reagents. did you find anything on their website? I found almost every detail
that I needed for my exp.
however, crosslinking is a tricky thing in that it varies from protein to
protein, and the condition for crosslinking matters a lot too. if you are
doing it in the presence of crude cell lysate, i doubt if there is established
protocols to follow. you probably need to try different crosslinking reagent,
different concentrat
reagents. did you find anything on their website? I found almost every detail
that I needed for my exp.
however, crosslinking is a tricky thing in that it varies from protein to
protein, and the condition for crosslinking matters a lot too. if you are
doing it in the presence of crude cell lysate, i doubt if there is established
protocols to follow. you probably need to try different crosslinking reagent,
different concentrat