Re: 请教！# Biology - 生物学
n*a
1 楼
Yeast 2 hybrid can test protein--protein interaction( based on modular nature
of activator/ suppressors):
proteinA proteinB+(AD) +
(BD)
of activator/ suppressors):
proteinA proteinB+(AD) +
(BD)
M*e
2 楼
analyze the homologous genes already known from AT genome to
see what people have done. get the most promising ones for
your project.
you have to verify the subcellular localization of these proteins
(in situ, western, IHC, IF, etc). establish developmental as
well as tissue-specific expression profiles for RNA/protein.
find out whether the expression or localization of these
proteins are induced, and by what kind of conditions. if so,
as you already suggested, investigate their functions under
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