Re: 请教RT-PCR的结果怎样是在线性范围内？# Biology - 生物学
s*e
1 楼
no,
however,
I am not sure whether you are talking about quantitative real-time PCR
or reverse transcription PCR.
For real-time PCR, no matter how many cycles you run, you are caculating
from the cycle number that first give a significant PCR signal. So
it doesn't related with your total PCR cycles. Although you may set your
PCR reaction to 35 cycles, if your PCR give a signal at 18 cycle number,
that is linear before 18 cycles.
For reverse transcription PCR, if you care about linear range, don'
however,
I am not sure whether you are talking about quantitative real-time PCR
or reverse transcription PCR.
For real-time PCR, no matter how many cycles you run, you are caculating
from the cycle number that first give a significant PCR signal. So
it doesn't related with your total PCR cycles. Although you may set your
PCR reaction to 35 cycles, if your PCR give a signal at 18 cycle number,
that is linear before 18 cycles.
For reverse transcription PCR, if you care about linear range, don'