Re: 跨膜蛋白的orientation?# Biology - 生物学
s*e
1 楼
(1)
fusiong protein with a enzyme digestion site. overexpress it and digest.
check media by western.
(2)
EGFP fused to N tel. microscopy
(3)
homology search of the N-ter from database.
(4)
I don't know. :)
fusiong protein with a enzyme digestion site. overexpress it and digest.
check media by western.
(2)
EGFP fused to N tel. microscopy
(3)
homology search of the N-ter from database.
(4)
I don't know. :)