Re: Apoptosis---Re: Call for MCBJC dicus - 未名空间MITBBS历史存档

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Re: Apoptosis---Re: Call for MCBJC dicus

Re: Apoptosis---Re: Call for MCBJC dicus# Biology - 生物学

M*e2003-04-05 08:04

1 楼

i have just read the paper and found it is more interesting than i have
expected. they basically took a chemical screening/biochemical analysis
approach to identify novel genes that regulate the activation of caspase-3.
they found a chemical compound, namely PETCM, that could promote apoptosis,
then they fractionated protein extracts and looked for proteins that mediated
the apoptome promotion effect of PETCM; and they characterized several PHAP
genes that are tumor suppressors as well as ProT w

l*k2003-04-05 08:04

2 楼

It is an amazing paper, proof of principle of HTS methods for drug screening
and deciphering
complicated signal transduction network.
However, I don't get it when they say: "In contrast, the presense of PHAPI did
not affect ... and
increased caspase-9 was associated with apoptosome" This should be the natural
function of PHAPI, right? and did they do a timecourse? as to if add PHAPI
first, then
ProT later, will this be reversed? I probably missed something here.
and why they only tested PHAP1, I

【在 M****e 的大作中提到】

: i have just read the paper and found it is more interesting than i have
: expected. they basically took a chemical screening/biochemical analysis
: approach to identify novel genes that regulate the activation of caspase-3.
: they found a chemical compound, namely PETCM, that could promote apoptosis,
: then they fractionated protein extracts and looked for proteins that mediated
: the apoptome promotion effect of PETCM; and they characterized several PHAP
: genes that are tumor suppressors as well as ProT w