尝试分析一下SARS# Biology - 生物学
g*m
1 楼
看看现有的database能够弄清哪些程度上的东西.
首先,不考虑codon preference条件下用NCBI的ORF
finder找到8个ORFs(P1-->P6),blastP结果如下:
检索ID:1050437955-05057-3024
P1 +1 ORF:250..13398 4383aa
bovine coronavirus RNA-directed RNA replicase
P2 +3 ORF:13584..21470 2629aa
同上,可能形成polyprotein complex
P3 +3 ORF:21477..25244 1256aa
surface projection glycoprotein of coronavirus
envelope protein
P4 +1 ORF:28105..29373 423aa
murine hepatitis virus nucleocapsid protein
P5 +2 ORF:25253..26077 275aa
Opsin, or malaria parasite Ca ATPase-like
首先,不考虑codon preference条件下用NCBI的ORF
finder找到8个ORFs(P1-->P6),blastP结果如下:
检索ID:1050437955-05057-3024
P1 +1 ORF:250..13398 4383aa
bovine coronavirus RNA-directed RNA replicase
P2 +3 ORF:13584..21470 2629aa
同上,可能形成polyprotein complex
P3 +3 ORF:21477..25244 1256aa
surface projection glycoprotein of coronavirus
envelope protein
P4 +1 ORF:28105..29373 423aa
murine hepatitis virus nucleocapsid protein
P5 +2 ORF:25253..26077 275aa
Opsin, or malaria parasite Ca ATPase-like