Re: Q about transfected protein localization# Biology - 生物学
m*o
1 楼
It looks the sepecificity of subcellular localization was modified. Did you
make any change to the N terminal of the protein? One possibility is the
specific signal pepetides at the N terminal has been modified that led to
the changes of its subcellular localization. Even the tag sequence may make
some changes. Well, sometime, in some case, the modification of subcellular
location may be seen from an overexpression cell line, that's possibly caused
by diffusion of either protein location or det
make any change to the N terminal of the protein? One possibility is the
specific signal pepetides at the N terminal has been modified that led to
the changes of its subcellular localization. Even the tag sequence may make
some changes. Well, sometime, in some case, the modification of subcellular
location may be seen from an overexpression cell line, that's possibly caused
by diffusion of either protein location or det