Re: recombinant蛋白质表达问题# Biology - 生物学
j*o
1 楼
Yes. Not only BL21codon plus, but almost all BL21s except BL21(DE3)pLysS/E are
leaky. pLysS/E encode enzyme that degrade T7 polymerase to avoid leaky.
In your case with many rare Arg codons, you have to use codon plus. So, it is
importment to avoid leaky? otherwise, just express with leaky if it does no
harm.
leaky. pLysS/E encode enzyme that degrade T7 polymerase to avoid leaky.
In your case with many rare Arg codons, you have to use codon plus. So, it is
importment to avoid leaky? otherwise, just express with leaky if it does no
harm.