Re: 请教一个蛋白质晶体结构的问题# Biology - 生物学
c*r
1 楼
In my experinces ( I have grown more than 15 crystals of more than 10
proteins), 1-5 mg can give a good chance. Usually people first try
screening kits (there are several kinds of kits), and if you are lucky
enough, you can get good crystals at the first trial. In this case,
you only need 1-2 mg protein.
If you can not find condition by screening, or you need to optimize
the conditions that give bad crstals, it will difficulty to say, but 5-10
mg can give very good chance.
But some proteins, rea
proteins), 1-5 mg can give a good chance. Usually people first try
screening kits (there are several kinds of kits), and if you are lucky
enough, you can get good crystals at the first trial. In this case,
you only need 1-2 mg protein.
If you can not find condition by screening, or you need to optimize
the conditions that give bad crstals, it will difficulty to say, but 5-10
mg can give very good chance.
But some proteins, rea