Re: How to do a western on insoluble protein# Biology - 生物学
C*n
1 楼
the more harsh your solution, the more components will disolve, of course the
more you will see on the gel.
If you want to disolve all the pellet, try the most "harsh" solution you can
imagine, like 5%-Triton-2%SDS-8M urea.....
more you will see on the gel.
If you want to disolve all the pellet, try the most "harsh" solution you can
imagine, like 5%-Triton-2%SDS-8M urea.....