Re: PCR primer question# Biology - 生物学
t*s
1 楼
I think different softwares score primers differently. But i don't think
they can be too much off each other.
Primers are dirt cheap. Why don't you design 2 pairs out of a software
and do an annealing temperature optimization? Or maybe run touch-down PCR.
RE map is restriction enyme map. You cut your PCR products with "RE"
and run on a gel to see if the cut produces correct DNA fragment bands.
they can be too much off each other.
Primers are dirt cheap. Why don't you design 2 pairs out of a software
and do an annealing temperature optimization? Or maybe run touch-down PCR.
RE map is restriction enyme map. You cut your PCR products with "RE"
and run on a gel to see if the cut produces correct DNA fragment bands.