Interesting report in Nature# Biology - 生物学
B*i
1 楼
下周一几个美国朋友请我去饭店吃饭。
1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
需要付小费么？
2. 如果去自助，需要付小费么？我看网上有说要有说不要的
谢谢
1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
需要付小费么？
2. 如果去自助，需要付小费么？我看网上有说要有说不要的
谢谢
M*y
2 楼
看到置顶的帖子说收费问题，想起来那张额度才3000的卡一直都没用了。。
因为另一张是forward基本覆盖了我主要的购物需要
这张该关掉么？
因为另一张是forward基本覆盖了我主要的购物需要
这张该关掉么？
s*y
3 楼
Finally! Somebody put a microscope inside the cell incubator and show that
this setting is possible. Continuous imaging of weeks is now possible.
Comopanies are also designing cell incubators coupled to microscope now.
http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
Cellular imaging: Taking a long, hard look
this setting is possible. Continuous imaging of weeks is now possible.
Comopanies are also designing cell incubators coupled to microscope now.
http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
Cellular imaging: Taking a long, hard look
g*y
4 楼
what's the occasion?是有人生日吗？
如果有什么特别要庆祝的，你就带礼物去好勒－－如果他明确给你说请你吃饭的话。
date的话，就直接去什么都不要准备，想吃什么就点，:))
【在 B*******i 的大作中提到】
: 下周一几个美国朋友请我去饭店吃饭。
: 1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
: 需要付小费么？
: 2. 如果去自助，需要付小费么？我看网上有说要有说不要的
: 谢谢
如果有什么特别要庆祝的，你就带礼物去好勒－－如果他明确给你说请你吃饭的话。
date的话，就直接去什么都不要准备，想吃什么就点，:))
【在 B*******i 的大作中提到】
: 下周一几个美国朋友请我去饭店吃饭。
: 1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
: 需要付小费么？
: 2. 如果去自助，需要付小费么？我看网上有说要有说不要的
: 谢谢
w*r
5 楼
这个早就实现了吧
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
z*h
6 楼
想太多
如果别人请你，你就什么都不用管了，下次请回来就好
给不给小费的规律非常简单，凡是吃完再给钱的都要给小费，先给钱后吃的就不用
【在 B*******i 的大作中提到】
: 下周一几个美国朋友请我去饭店吃饭。
: 1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
: 需要付小费么？
: 2. 如果去自助，需要付小费么？我看网上有说要有说不要的
: 谢谢
如果别人请你，你就什么都不用管了，下次请回来就好
给不给小费的规律非常简单，凡是吃完再给钱的都要给小费，先给钱后吃的就不用
【在 B*******i 的大作中提到】
: 下周一几个美国朋友请我去饭店吃饭。
: 1.如果是分开账单则没问题大家自己付自己的，但是他告诉我说他要COVER，这样我还
: 需要付小费么？
: 2. 如果去自助，需要付小费么？我看网上有说要有说不要的
: 谢谢
a*d
7 楼
time lapse study of cell lineage is really powerful, and we sure need more
advanced tools to get into more details
advanced tools to get into more details
n*n
8 楼
Just real time-lapse.
p*r
9 楼
We have been doing this in our lab for years...
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
s*r
10 楼
怎么知道放了那个microscope的细胞在各种生理条件下都和没放的细胞一样
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
T*t
11 楼
为什么说finally啊，那篇文章里，最早的尝试在80年代就开始了。
不过我之前倒是不知道在incubator里放个显微镜或者在显微镜下放个incubator原来还
有这么多学问。长知识了。
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
不过我之前倒是不知道在incubator里放个显微镜或者在显微镜下放个incubator原来还
有这么多学问。长知识了。
【在 s******y 的大作中提到】
: Finally! Somebody put a microscope inside the cell incubator and show that
: this setting is possible. Continuous imaging of weeks is now possible.
: Comopanies are also designing cell incubators coupled to microscope now.
: http://www.nature.com/nature/journal/v466/n7310/full/4661137a.html
: Cellular imaging: Taking a long, hard look
s*y
12 楼
Really?
Well, we had had similar attempt before but the water somehow condense onto
the lens chamber and made a big mess out of it. So we decided not to try it
again :)
If you are talking about those enclosed chambers over the observation
platform, we have that too. However it can only be used to maintain
temperature, and humidity to a certain extend. It was unable to used for CO2
maintenance because it not air tight.
The one in this report is a microscope completely inside a cell incubator.
【在 p*******r 的大作中提到】
: We have been doing this in our lab for years...
Well, we had had similar attempt before but the water somehow condense onto
the lens chamber and made a big mess out of it. So we decided not to try it
again :)
If you are talking about those enclosed chambers over the observation
platform, we have that too. However it can only be used to maintain
temperature, and humidity to a certain extend. It was unable to used for CO2
maintenance because it not air tight.
The one in this report is a microscope completely inside a cell incubator.
【在 p*******r 的大作中提到】
: We have been doing this in our lab for years...
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