Help! Transfer HMW protein (360kDa) from SDS-PAGE to PVDF membrane# Biology - 生物学
c*l
1 楼
刚写了一本书。请问书的版权问题应该怎样和出版社协商？是不是书卖出的盈利都归出
版社？
版社？
D*a
2 楼
黑圈里面
g*1
3 楼
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
b*d
4 楼
他应该会给你incentive.卖出去一本，应该给你些钱。
y*8
5 楼
4-5% resolving gel and transfer buffer without methanol.
U*1
6 楼
read your contract
v*a
7 楼
without methanol是什么原理啊？
【在 y******8 的大作中提到】
: 4-5% resolving gel and transfer buffer without methanol.
【在 y******8 的大作中提到】
: 4-5% resolving gel and transfer buffer without methanol.
s*r
8 楼
是怎么和出版社谈合同的？有什么经验
【在 c**l 的大作中提到】
: 刚写了一本书。请问书的版权问题应该怎样和出版社协商？是不是书卖出的盈利都归出
: 版社？
【在 c**l 的大作中提到】
: 刚写了一本书。请问书的版权问题应该怎样和出版社协商？是不是书卖出的盈利都归出
: 版社？
i*e
9 楼
I use 6% separation gel and transfer without methanol for one hour at 100 V
. My protein is 550 KDa.
. My protein is 550 KDa.
s*r
10 楼
invirogen 有 Tis-acetate gel，据广告适合大蛋白。
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
【在 g***1 的大作中提到】
: Does anyone have experiences in western blot handling high molecular weight
: (250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
: sharing your experiences!
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
【在 g***1 的大作中提到】
: Does anyone have experiences in western blot handling high molecular weight
: (250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
: sharing your experiences!
i*t
11 楼
add SDS in trnasfer buffer.
p*t
12 楼
Are you labeling Ryanoding Receptor?
V
【在 i********e 的大作中提到】
: I use 6% separation gel and transfer without methanol for one hour at 100 V
: . My protein is 550 KDa.
V
【在 i********e 的大作中提到】
: I use 6% separation gel and transfer without methanol for one hour at 100 V
: . My protein is 550 KDa.
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