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reversible protein precipitate with temperature

reversible protein precipitate with temperature# Biology - 生物学

P*y2011-03-18 07:03

1 楼

比如说在半导体行业，如果做半导体wire bond有5年经验。现在想换工作。请问是接着
做本行比较好呢，还是去做相关的Die Bond什么的？
说白了就是到底是一条路走到黑呢，还是相关方面都有一些经历比较好。
恳请各位放开讨论。谢谢。

x*i2011-03-18 07:03

2 楼

Hi there,
We have a protein that is soluble at room temperature but precipitates after
placing in refrigerator overnight. This is a reversible process, e.g.,
soluble again with temperature increase to room temperature.
Appreciate any idea or any possible explanation for it. What experiment
could be done to verify any hypothesis?
Thanks

n*t2011-03-18 07:03

3 楼

不可能相差太大的
即使你愿意，公司也不会愿意招一个完全没有相关经验的

z*h2011-03-18 07:03

4 楼

The simplest explanation is solubility vs temperature.

h*y2011-03-18 07:03

5 楼

专一点好

s*s2011-03-18 07:03

6 楼

啥buffer呀？
化学里面也就一个溶解自由能的大小

after

【在 x*******i 的大作中提到】

: Hi there,
: We have a protein that is soluble at room temperature but precipitates after
: placing in refrigerator overnight. This is a reversible process, e.g.,
: soluble again with temperature increase to room temperature.
: Appreciate any idea or any possible explanation for it. What experiment
: could be done to verify any hypothesis?
: Thanks