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Early sign of chimera mice that carrying gremlin transmission.
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Early sign of chimera mice that carrying gremlin transmission.# Biology - 生物学
p*i
1
http://blogs.greatschools.org/greatschoolsblog/2010/10/bullying-that-ends-in-suicide.html
October 14, 2010
Jon Carmichael, age 13
Hope Witsell, age 13
Justin Aaberg, age 15
Asher Brown, age 13
Seth Walsh, age 13
Phoebe Prince, age 15
Billy Lucas, age 15
All of these children — from places like Houston; Tehachapi, Calif.; and
Rushkin, Fla. — killed themselves this year. Each was a victim of bullying,
or what is now called “bullicide.” Many of these teens were also victims
of anti-gay hate crimes.
Here we are in October — National Bullying Prevention Month — and rarely
have we seen, directly as a result of bullying, such a high toll of teenage
suicides in less than a year. What’s so confounding is that now, more than
ever, there’s ramped-up awareness about bullying, more anti-bullying
programs in schools across the country, and more anti-bullying legislation
passed in 45 states.
Who do we blame now that so many bullied kids are taking their lives? The
bullies who continue their abuse unchecked? Negligent school administrators?
Lax state and federal laws? Adults who aren’t taking kids’ and parents’
complaints seriously enough?
Read news reports following most any of these kids’ suicides. The victims’
parents often echo one another, saying that their school’s administration
didn’t take strong action following repeated complaints. This is the case
with 13-year-old Asher Brown, whose parents said he was “'bullied to death
’ — picked on for his small size, his religion and because he did not wear
designer clothes and shoes. Kids also accused him of being gay, some of
them performing mock gay acts on him in his physical education class… The
13-year-old's parents said they had complained about the bullying to
Hamilton Middle School officials during the past 18 months, but claimed
their concerns fell on deaf ears.”
Or, despite undergoing anti-bullying training at Jacobsen Middle School in
Tehachapi, administrators still didn’t put an end to the bullying of 13-
year-old Seth Walsh.
(Imagine going to work and, day after day, having office mates send out
humiliating and threatening emails to the entire staff and tripping and
punching you as you walk by their cubicles. And continuing to have to show
up, without anyone putting an end to your physical and emotional abuse.)
How can we save bullied kids’ lives? Columnist Dan Savage has done his part
for GLBT (gay, lesbian, bixexual, and transgender) kids by launching the It
Gets Better Project, with grown-ups giving testimonies that, once they left
school, life got better for them. (There are compelling reasons to directly
reach out to GLBT kids: Nine out of 10 GLBT students have experienced
harassment at school and are bullied two to three times as much as straight
teens. More than one-third of GLBT kids have attempted to commit suicide and
are four times as likely to attempt suicide then their straight peers.)
There’s hope that with a strong enough no-tolerance campaign for bullying,
such abuse will virtually end. A friend who is familiar with the schools in
Norway has told me that’s what happened in that country. Home of the
original anti-bullying program, Olweus, which is being implemented in many U
.S. schools, bullying there is far less common, just through the sheer force
of creating a zero-tolerance anti-bullying culture.
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z*u
2
想海运寄一点包裹回去, usps 还有 fedex 都很贵。请问有什么便宜的海运方法?怎
么办理?费用如何?谢谢。
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j*l
3
有钱的人的生活一直都是我自己追求的目标,我的生活当中大多数的时间都在研究着让
自己怎样才能成为一个富贵的人。每天当自己走在大街上面的时候,自己见到的最多的
就是各种各样介绍你怎样发财的书籍,但是自己从来就没有真正的看过,或许那个时候
的自己还没有那莫大的动力的原因。后来因为物质上的匮乏,让我自己开始慢慢的对金
钱有了很大的渴望,渴望自己可以能够更好的生活下去,但是要是想要更好的生活下去
的话,最大的关键就是要知道怎样才能更快更好的赚钱才是最主要的事情。以前的时候
自己最喜欢干的事情就是各种各样的玩耍,但是那个时候的自己还小,但是现在的自己
就不同了。现在的我虽然不是很富有,但是最起码的自己已经有了想要成为富翁的梦想
。虽然自己现在还不值一提,但是我也不能放弃自己的梦想,每当从车窗里面看着富人
的悠闲生活的时候自己心中最大的感受就是羡慕,希望自己的某一天也可以能够开上自
己想要的豪车,拥有一个自由身而不是被工作束缚住的人。
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o*y
4
凡是 PM 了邮寄地址给我的,今天都给你们寄了,注意查收。
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y*u
5
Early sign of chimera mice that carrying gremlin transmission.
Dear all, I am in a hurry to decide if it is worth to inject another ES cell
line of a KO mice.
The ES cell line I am using is SV129 cell line ,which carrying agouti coat
colour.
Right now I am having 9 male chimera mice and 1 female chimera mice, all of
the male chimera mice are with very high agouti number indicating a strong
ES cell contribution, and also, as stated above, a distorted gender ratio.
Meantime , I am getting above 80% of normal karyotype
Is there any early sign we can count on to determine the possibility of ES
cell germ line contribution?
Some people told me that regardless how good the early sign is, it is still
possible to have all the male mice failed to give out germline transmission
, which is very disturbing.
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y*u
6
waiting^^^^^
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m*5
7
我靠,我和LZ有一样的问题,我都panic了
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i*0
8
normally we inject several clones and then backcross to b6 and wait and
confirm with southern or PCR.
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m*5
9
不知道楼主的情况。。我是因为冰箱事故只有一个克隆活下来

【在 i*********0 的大作中提到】
: normally we inject several clones and then backcross to b6 and wait and
: confirm with southern or PCR.

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y*u
10
我们也没好到哪里去,8个克隆只有一个karyotype正常,7个是7号tri
somy

【在 m******5 的大作中提到】
: 不知道楼主的情况。。我是因为冰箱事故只有一个克隆活下来
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m*5
11
你们是不是一开始挑了特别大的克隆……

【在 y*********u 的大作中提到】
: 我们也没好到哪里去,8个克隆只有一个karyotype正常,7个是7号tri
: somy

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m*5
12
理论上说那种特别大的colony很有可能是trisomy 8

【在 m******5 的大作中提到】
: 你们是不是一开始挑了特别大的克隆……
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m*5
13
楼主消失了……但我还是很想知道有没有定论……
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