information on nucleotides around restriction enzyme - 未名空间MITBBS历史存档

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information on nucleotides around restriction enzyme

information on nucleotides around restriction enzyme# Biology - 生物学

f*y2011-07-03 07:07

1 楼

如今有很多出入职场的人被称之为职场菜鸟，那么职场菜鸟应该具备什么样的特征
才能够快速的升职呢？下面来说一说。
第一，出入职场的菜鸟应该学会发展自己的人脉，因为一个公司好的人缘和好的人
脉是非常重要的，因为菜鸟刚开始进入公司不是什么都懂，所以需要良好的人脉帮助菜
鸟渡过这个适应期和熟悉期。第二，要有一个好的人品，俗话说看人看人品，所以说，
一个人的人品对于工作和升职也是非常重要的。第三，勤学苦练，不放弃充电的机会。
这样可以让领导们看到菜鸟的勤奋，并且能够给菜鸟们更多的机会。
职场对于菜鸟来说，是一个既陌生又渴望熟悉的新环境，作为职场的菜鸟，没有任
何的背景，但是对于职场不能够没有憧憬，一个人可以没有远大的理想，但是一定要有
自己的思想，所以菜鸟们都应该行动起来，做好自己的本职工作，并且能够做到以上的
三点，让自己能够被人看到积极勤奋的一面，同时也要培养自己的性格，让自己的人品
更加的高贵，让自己成为潜力股。

T*N2011-07-03 07:07

2 楼

http://www.edn.com/blog/Now_Hear_This_/41259-HP_TouchPad_fire_s

t*12011-07-03 07:07

3 楼

I am trying to clone a promoter sequence to a Vector. Now I have a question
on designing primers. I want to add a restriction enzyme at the end of each
primer, and I also heard that it is better to add a few bp around the
restriction enzyme for it to be cleaved. Does anyone know where I can find
such information on which nucleotide to be added to different restriction
enzyme?
(I read New England catalog book and did not find such information.)
Thank you!

b*n2011-07-03 07:07

4 楼

The old catalog has the information, but it is removed somehow.
Usually, just add GGTGGT to the 5' of the restriction site.

question
each

【在 t*********1 的大作中提到】

: I am trying to clone a promoter sequence to a Vector. Now I have a question
: on designing primers. I want to add a restriction enzyme at the end of each
: primer, and I also heard that it is better to add a few bp around the
: restriction enzyme for it to be cleaved. Does anyone know where I can find
: such information on which nucleotide to be added to different restriction
: enzyme?
: (I read New England catalog book and did not find such information.)
: Thank you!