MDA-MB-231生成肿瘤失败 - 未名空间MITBBS历史存档

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MDA-MB-231生成肿瘤失败

MDA-MB-231生成肿瘤失败# Biology - 生物学

b*e2011-07-21 07:07

1 楼

我现在在一个几千人的上市公司做一线开发经理，考虑跳槽，收到两个offer.一个是一
个二百多人的小公司（有盈利好几年了，被大私募公司leverage buyout 收购2年了，
独立运营）的director的职位，
会管理两个团队，第一个团队在中国，有个一线经理在中国直接管理，第二个团队有4
个人在美国而且没有一线经理，我需要直接管理美国的这四个人。估计自己参与开发的
比重不大。
另一个offer是一个一千人的公司的senior manager职位，管理一个不到7个人的美国团
队，这个公司没有上市。我自己也得参与开发。
工资待遇都差不多。请问从职业发展的角度如何选择，有何利弊？
1）是不是小公司的管理经验不被大公司重视，以后再跳槽大公司的管理职位有难度？
以后还是很想去大公司做管理的。
2）是不是这俩个offer 都不好，就留在现在这个几千人的上市公司做一线经理。
多谢回复

Z*e2011-07-21 07:07

2 楼

全集那种

y*62011-07-21 07:07

3 楼

最近用MDA-MB-231 cell 在裸鼠皮下生成肿瘤失败(s.c.injection on back, male
nude mice, 5 million cells in 100 ul DMEM w/o serum, no tumor after >6 weeks
)。同样条件SK-OV-3可以产生肿瘤。我印象中MDA-MB-231比SK-OV-3容易生成肿瘤。有
经验的请说说可能问题出在那里？不会因为MDA-MB-231在公鼠上不长吧。

a*g2011-07-21 07:07

4 楼

return china langfang

4

【在 b*********e 的大作中提到】

: 我现在在一个几千人的上市公司做一线开发经理，考虑跳槽，收到两个offer.一个是一
: 个二百多人的小公司（有盈利好几年了，被大私募公司leverage buyout 收购2年了，
: 独立运营）的director的职位，
: 会管理两个团队，第一个团队在中国，有个一线经理在中国直接管理，第二个团队有4
: 个人在美国而且没有一线经理，我需要直接管理美国的这四个人。估计自己参与开发的
: 比重不大。
: 另一个offer是一个一千人的公司的senior manager职位，管理一个不到7个人的美国团
: 队，这个公司没有上市。我自己也得参与开发。
: 工资待遇都差不多。请问从职业发展的角度如何选择，有何利弊？
: 1）是不是小公司的管理经验不被大公司重视，以后再跳槽大公司的管理职位有难度？

l*r2011-07-21 07:07

5 楼

请给包子吧，
http://pan.baidu.com/s/1hq3Lz5U
320K MP3

q*n2011-07-21 07:07

6 楼

Why not try female mice?
MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
group have developed some derivative cell lines, such as LM2, which grows
faster and metastasize better. here is the reference:
Nature. 2005 Jul 28;436(7050):518-24.
Genes that mediate breast cancer metastasis to lung.
Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
Gerald WL, Massagué J.
You can write and request LM2 cells and they will give for free.

Z*e2011-07-21 07:07

7 楼

thx

【在 l*****r 的大作中提到】

: 请给包子吧，
: http://pan.baidu.com/s/1hq3Lz5U
: 320K MP3

A*y2011-07-21 07:07

8 楼

Use matrigel for consistency, and expect about 2 out 10 mice won't even
engraft. You also need about 10 million cells per injection.

y*62011-07-21 07:07

9 楼

Thanks a lot for the information. However I already made a stable cell line
from the original MDA-MB-231, which means I cannot use the derivatives. I
will try female mice, and inject more cells (as suggested by "ArtyArty").
MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
group have developed some derivative cell lines, such as LM2, which grows
faster and metastasize better. here is the reference:
Nature. 2005 Jul 28;436(7050):518-24.
Genes that mediate breast cancer metastasis to lung.
Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
Gerald WL, Massagué J.

m*82011-07-21 07:07

10 楼

Female nude rat modeling can be succeeded at 80% tumor rate about 3 weeks
post 6M cells in 0.2 ml saline were injected orthotopically.

D*92011-07-21 07:07

11 楼

I used 2X105 231 cells, developed tumors in 4 weeks.There's something wrong
with your cells.Try to get a new vial from other lab.

y*62011-07-21 07:07

12 楼

Do you inject at the mammary gland? I do not know whether that makes
difference. Thanks.
I used 2X105 231 cells, developed tumors in 4 weeks.There's something wrong
with your cells.Try to get a new vial from other lab.

D*92011-07-21 07:07

13 楼

no you don't have to. actually mouse and human breast structures are very
different. you can not mimic human breast environment in mouse.

D*92011-07-21 07:07

14 楼

MDA231 is a triple-negative breast cancer cell, very aggressive. The size of
needle matters, do not use too narrow needle because it may hurt the cells.

g*02011-07-21 07:07

15 楼

May I ask for the best ref for your statement? Thanks,

【在 D******9 的大作中提到】

: no you don't have to. actually mouse and human breast structures are very
: different. you can not mimic human breast environment in mouse.

c*t2011-07-21 07:07

16 楼

你是稳定转染的细胞株？敲掉/加入了什么基因？没处理之前的细胞株长不长？
如果只做过一次不能确定不能成瘤。比方消化时间过久也可能。
这个细胞系应该很容易做。绝对不要等6个礼拜。

line
derivatives. I
"ArtyArty").
Massague'
grows
AB,

【在 y****6 的大作中提到】

: Thanks a lot for the information. However I already made a stable cell line
: from the original MDA-MB-231, which means I cannot use the derivatives. I
: will try female mice, and inject more cells (as suggested by "ArtyArty").
: MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
: group have developed some derivative cell lines, such as LM2, which grows
: faster and metastasize better. here is the reference:
: Nature. 2005 Jul 28;436(7050):518-24.
: Genes that mediate breast cancer metastasis to lung.
: Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
: Gerald WL, Massagué J.

y*62011-07-21 07:07

17 楼

The parental cells do not grow tumors. So I believe something is wrong with
my system.

【在 c*******t 的大作中提到】

: 你是稳定转染的细胞株？敲掉/加入了什么基因？没处理之前的细胞株长不长？
: 如果只做过一次不能确定不能成瘤。比方消化时间过久也可能。
: 这个细胞系应该很容易做。绝对不要等6个礼拜。
:
: line
: derivatives. I
: "ArtyArty").
: Massague'
: grows
: AB,

a*g2011-07-21 07:07

18 楼

assume you have done mycoplasma test?

with

【在 y****6 的大作中提到】

: The parental cells do not grow tumors. So I believe something is wrong with
: my system.