【在 x********u 的大作中提到】 : 今天刚查了状态,是Request for Evidence。请问这个通常是什么问题?会耽误很久吗 : ?谢谢!
t*d
4 楼
参考一下这个: A major problem with validating RNA-Seq expression estimates is that there is no clear 'gold standard' for expression estimation. Comparison of RNA-Seq to microarrays has suggested that the former technology is more accurate than the latter. We examined the recently published NanoString nCounter gene expression system, but noticed many unexplainable outliers and high variance between technical replicates (see Figure S4 of Additional file 1 and data in Additional file 2). Quantitative reverse transcription PCR (qRT- PCR) has served as a benchmark in numerous studies but it is not a perfect expression measurement assay, and it is therefore a priori unclear which technology currently produces the most accurate expression estimates. Nevertheless, at present we believe it to be the best measure of expression aside from, perhaps, RNA-Seq itself. Due to the previously demonstrated superiority of RNA-Seq over microarrays, and the problems with NanoString, we performed all our benchmarking with respect to qRT-PCR. PMID: 21410973