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求junctional proteins (ZO-1, V-Cadherin, etc)抗体~谢谢~
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求junctional proteins (ZO-1, V-Cadherin, etc)抗体~谢谢~# Biology - 生物学
L*r
1
只扫了自己的号前后左右一些的,感谢leftHand大侠的code。
"1290147243 -I131, AP:Initial Review "
"1290147245 -I131, AP:Card/ Document Production "
"1290147266 -I765, EAD:Card/ Document Production "
"1290147312 -I765, EAD:Card/ Document Production "
"1290147319 -I485, GC:Initial Review "
"1290147320 -I131, AP:Initial Review "
"1290147324 -I485, GC:Initial Review "
"1290147325 -I131, AP:Initial Review "
"1290147350 -I765, EAD:Initial Review "
"1290147370 -I131, AP:Post Decision Activity "
"1290147438 -I765, EAD:Card/ Document Production "
"1290147464 -I765, EAD:Card/ Document Production "
"1290147465 -I765, EAD:Card/ Document Production "
"1290147469 -I765, EAD:Card/ Document Production "
"1290147479 -I765, EAD:Card/ Document Production "
"1290147483 -I765, EAD:Initial Review "
"1290147547 -I131, AP:Post Decision Activity "
"1290147568 -I765, EAD:Card/ Document Production "
"1290147596 -I765, EAD:Initial Review "
"1290147598 -I131, AP:Initial Review "
"1290147612 -I765, EAD:Card/ Document Production "
"1290147639 -I765, EAD:Card/ Document Production "
"1290147654 -I765, EAD:Card/ Document Production "
"1290147657 -I765, EAD:Card/ Document Production "
"1290147661 -I765, EAD:Initial Review "
"1290147664 -I765, EAD:Card/ Document Production "
"1290147668 -I765, EAD:Card/ Document Production "
"1290147669 -I765, EAD:Card/ Document Production "
"1290147690 -I765, EAD:Card/ Document Production "
"1290147750 -I765, EAD:Initial Review "
"1290147772 -I765, EAD:Card/ Document Production "
"1290147786 -I765, EAD:Card/ Document Production "
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n*w
2
最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC
都不行。
版上有人做过吗?
有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢
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S*n
3
请问这个区间RD是多少?THX!!
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s*y
4
Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。
对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。
注意要买这个单抗的。他们的那个多抗的质量太不稳定了,可把我坑苦了!
前一阵子还上来诉苦的。

IHC

【在 n***w 的大作中提到】
: 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
: 试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC
: 都不行。
: 版上有人做过吗?
: 有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢

avatar
p*e
5
Zo1我们用invitrogen Mouse的,还不错,但是兔子的不work.

IHC

【在 n***w 的大作中提到】
: 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
: 试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC
: 都不行。
: 版上有人做过吗?
: 有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢

avatar
n*w
6
前面没说清楚,
我就是要染老鼠tissue,所以我尽量避免mouse mAb...

【在 p*****e 的大作中提到】
: Zo1我们用invitrogen Mouse的,还不错,但是兔子的不work.
:
: IHC

avatar
n*w
7
谢谢嗯,我是想染whole retina嗯,所以避免单抗。。

【在 s******y 的大作中提到】
: Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。
: 对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。
: 注意要买这个单抗的。他们的那个多抗的质量太不稳定了,可把我坑苦了!
: 前一阵子还上来诉苦的。
:
: IHC

avatar
s*y
8
oh....那就没办法了。。。
Sigma 也有Rabbit anti PAN cadherin,但是是个多抗,每一批都不一样!
倒是每次都能做WB 是没有问题的,染经典的E-cadherin 也每批都行,
就是染N, VE 不是每批都行

【在 n***w 的大作中提到】
: 前面没说清楚,
: 我就是要染老鼠tissue,所以我尽量避免mouse mAb...

avatar
p*e
9
那就不知道了,不好意思,我也是初学者

【在 n***w 的大作中提到】
: 前面没说清楚,
: 我就是要染老鼠tissue,所以我尽量避免mouse mAb...

avatar
g*y
10
我们用很多mouse antibody 染老鼠tissue...

【在 n***w 的大作中提到】
: 前面没说清楚,
: 我就是要染老鼠tissue,所以我尽量避免mouse mAb...

avatar
n*w
11
嗯,你们是怎么处理的?因为我要染血管,所以比较担心。。。。
用no primary做对照?还是用了什么特别的kit,比如说vector labs的m.o.m?
谢谢。
真希望有些GFP junctional protein mice。

【在 g***y 的大作中提到】
: 我们用很多mouse antibody 染老鼠tissue...
avatar
g*y
12

we stain brain tissues.we perfuse mouse first
yes

【在 n***w 的大作中提到】
: 嗯,你们是怎么处理的?因为我要染血管,所以比较担心。。。。
: 用no primary做对照?还是用了什么特别的kit,比如说vector labs的m.o.m?
: 谢谢。
: 真希望有些GFP junctional protein mice。

avatar
l*1
13
RE: 德艺双馨的楼主
please go to
//www.creativebiomart.net/description_10243_28.htm
You Position: Home >> Antibody >> Polyclonal Antibody >> Rabbit PAb >> Anti-Cdh5 PAb
Anti-Cdh5 PAb
Cat. No.
CPB-133RM
>
Immunogen
Recombinant mouse CDH5 protein.
Antibody Type
Rabbit Polyclonal Antibody.
Ig Type
Rabbit IgG.
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Preparation
Produced in rabbits immunized with purified, human cell-derived, recombinant mouse CDH5 extracellular
domain (Met 1-Ala 592). Total IgG was purified by Protein A affinity chromatography.
Specificity
Mouse CDH5/VE-Cad/CD144.
Direct ELISA
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse
CDH5. The detection limit for mouse CDH5 is 0.0195 ng/well.
Storage
This antibody can be stored at 2oC-8oC for one month without detectable loss of activity. Antibody
products are stable for twelve months from date of receipt when stored at -20oC to -70oC. Preservative-
Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to
cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ps:
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//www.creativebiomart.net/

【在 n***w 的大作中提到】
: 谢谢嗯,我是想染whole retina嗯,所以避免单抗。。
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