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关于DNA聚合酶的问题
avatar
f*g
2 楼
DNA聚合酶每天都在不停的合成DNA，包括高等动物，低等动物，原核，真核。但是在不
停的合成中会产生突变。
我的问题是哪种DNA聚合酶的突变率最低?
到目前为止，to my knowledge, 能够买到的DNA聚合酶中，phusion的突变率是最低的
。有没有可能人的DNA聚合酶的突变率比phusion更低呢?
thank you
avatar
f*g
4 楼
ding
avatar
T*n
5 楼
kf的优势还是amazon的内容吧
root不过是撒点盐

【在 a*o 的大作中提到】
: 199够买好多其它强大得多的7吋板子了啊。
avatar
f*g
6 楼
ding
avatar
h*e
7 楼
for instance?

【在 a*o 的大作中提到】
: 199够买好多其它强大得多的7吋板子了啊。
avatar
f*g
8 楼
这个领域的xdjm给点文献也成啊
avatar
t*t
9 楼
vizio tablet

【在 h*e 的大作中提到】
: for instance?
avatar
f*g
10 楼
DNA聚合酶的核心doamin是什么?
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f*g
12 楼
ding
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K*4
14 楼
Well, I can not give you an answer to your question like 哪种DNA聚合酶的突变
率最低?
But from my friend's work, I can tell you that for DNA replication, many polymerases are involved, but only one is the primary enzyme. In the case of archaea and eukaryotes, this is Pol II (or better known as Family B DNA polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol III belong to distinct folds.
In nature, organisms dont need to maintain very reliable polymerases since allowing mutation during DNA synthesis is a need for the evolution, just like what viruses do. Thus I guess those commercial enzymes like phusion may have 突变率最低 since they have been optimized.

【在 f******g 的大作中提到】
: DNA聚合酶每天都在不停的合成DNA，包括高等动物，低等动物，原核，真核。但是在不
: 停的合成中会产生突变。
: 我的问题是哪种DNA聚合酶的突变率最低?
: 到目前为止，to my knowledge, 能够买到的DNA聚合酶中，phusion的突变率是最低的
: 。有没有可能人的DNA聚合酶的突变率比phusion更低呢?
: thank you
avatar
f*g
16 楼
thank you so much.

polymerases are involved, but only one is the primary enzyme. In the case of
archaea and eukaryotes, this is Pol II (or better known as Family B DNA
polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol
III belong to distinct folds.
allowing mutation during DNA synthesis is a need for the evolution, just
like what viruses do. Thus I guess those commercial enzymes like phusion may
have 突变率最低 since they have been optimized.

【在 K**4 的大作中提到】
: Well, I can not give you an answer to your question like 哪种DNA聚合酶的突变
: 率最低?
: But from my friend's work, I can tell you that for DNA replication, many polymerases are involved, but only one is the primary enzyme. In the case of archaea and eukaryotes, this is Pol II (or better known as Family B DNA polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol III belong to distinct folds.
: In nature, organisms dont need to maintain very reliable polymerases since allowing mutation during DNA synthesis is a need for the evolution, just like what viruses do. Thus I guess those commercial enzymes like phusion may have 突变率最低 since they have been optimized.
avatar
m*c
17 楼
199和amazon的招牌。

【在 o***t 的大作中提到】
: 这个东东的性价比究竟高在哪里？
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r*x
18 楼
新手弱问什么是SDK？ 另外，ROOT后会不会对电池什么的有影响啊？
avatar
m*z
19 楼
搭配prime，要不然不值。

【在 m********c 的大作中提到】
: 199和amazon的招牌。
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