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大蛋白的病毒包装问题
avatar
P*r
3
蛋白220kd,用lentivirus表达,但是做病毒的时候发现titer超低
板上有没有谁有心得的,分享一下。
avatar
h*k
4
应该是系统自己创建一个缺省的copy constructor,做shalow copy,就是直接拷贝每
个member。
avatar
z*n
5
不错,合音也是自己唱的?
avatar
r*r
6
太大了 也听了很多失败例子
avatar
m*n
7
A b; b = a; 好像不是copy constructor吧,又不是 A b = a; overload operator "=
"吧.
A & A::operator =(const A &other)
{
if(this == &other)
return *this;
const_cast(a) = other.a;
return *this;
}
avatar
c*u
8
呵呵,不是还是天籁的伴奏,这个风格其实很适合弹唱,可惜我的家伙全在国内,没带
过来

【在 z******n 的大作中提到】
: 不错,合音也是自己唱的?
avatar
n*k
9
In vitro or in vivo study?
try to cut down ur backbone as much as possible, dependent on which vector
you use, this could be like 12-15kb, anything above 11/12kb is a bit
struggle and above 13/15 is a big one...
BTW, not my personal experience but common knowledge from the field, the
longest oone I worked is about 12kb I believe...it was fine...not great
though

【在 P******r 的大作中提到】
: 蛋白220kd,用lentivirus表达,但是做病毒的时候发现titer超低
: 板上有没有谁有心得的,分享一下。

avatar
c*e
10
This is good. Thank you.

"=

【在 m*****n 的大作中提到】
: A b; b = a; 好像不是copy constructor吧,又不是 A b = a; overload operator "=
: "吧.
: A & A::operator =(const A &other)
: {
: if(this == &other)
: return *this;
: const_cast(a) = other.a;
: return *this;
: }

avatar
w*w
11
呵呵,今天下午还在马六甲K这歌来着,各种气不够用的说~~
唱的狠温柔,赞!
只有一小段?没听够~~
avatar
P*r
12
Thanks.

【在 n********k 的大作中提到】
: In vitro or in vivo study?
: try to cut down ur backbone as much as possible, dependent on which vector
: you use, this could be like 12-15kb, anything above 11/12kb is a bit
: struggle and above 13/15 is a big one...
: BTW, not my personal experience but common knowledge from the field, the
: longest oone I worked is about 12kb I believe...it was fine...not great
: though

avatar
c*u
13
ipad没电了,最后5%奋力一搏,充电中 ^_^

【在 w***w 的大作中提到】
: 呵呵,今天下午还在马六甲K这歌来着,各种气不够用的说~~
: 唱的狠温柔,赞!
: 只有一小段?没听够~~

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