j*1
2 楼
一个药物处理3天后,ATP含量增加了10-30倍,其实和对照比细胞数量要少10倍。也就
是说每个细胞ATP增加了100倍。这样的结果可信吗?我们用的是promega
Mitochondrial ToxGlo™ Assay.
是说每个细胞ATP增加了100倍。这样的结果可信吗?我们用的是promega
Mitochondrial ToxGlo™ Assay.
q*f
5 楼
costco的房子不便宜, 他们家车便宜
而且不知道为什么他们公司不肯帮我保房子
而且不知道为什么他们公司不肯帮我保房子
A*y
6 楼
Don't forget, cellular ATP source is from the adenine pool. Some cells has
90% of adenine in ATP normally. Therefore, how can you increase your ATP
level by 10-30 folds? How about you just do the ATP step? Did you check
your standard curve?
Also, if you have ATP increases, why your cells are died?
【在 j******1 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: 一个药物处理3天后,ATP含量增加了10-30倍,其实和对照比细胞数量要少10倍。也就
: 是说每个细胞ATP增加了100倍。这样的结果可信吗?我们用的是promega
: Mitochondrial ToxGlo™ Assay.
90% of adenine in ATP normally. Therefore, how can you increase your ATP
level by 10-30 folds? How about you just do the ATP step? Did you check
your standard curve?
Also, if you have ATP increases, why your cells are died?
【在 j******1 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: 一个药物处理3天后,ATP含量增加了10-30倍,其实和对照比细胞数量要少10倍。也就
: 是说每个细胞ATP增加了100倍。这样的结果可信吗?我们用的是promega
: Mitochondrial ToxGlo™ Assay.
j*1
7 楼
I doubt this results too, but the data is repeatable, so I want to try
another assay to confirm it.
another assay to confirm it.
A*y
8 楼
You ATP kit is the luciferase kit. If you want stable signal try this kit (
less sensitive but stable):
http://www.biovision.com/atp-colorimetric-fluorometric-assay-ki
Btw, the gold standard for ATP/ADP/AMP determination is the HPLC method.
【在 j******1 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: I doubt this results too, but the data is repeatable, so I want to try
: another assay to confirm it.
less sensitive but stable):
http://www.biovision.com/atp-colorimetric-fluorometric-assay-ki
Btw, the gold standard for ATP/ADP/AMP determination is the HPLC method.
【在 j******1 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: I doubt this results too, but the data is repeatable, so I want to try
: another assay to confirm it.
h*o
9 楼
个人觉得至少要做一个ATP/ADP ratio
最好能有ATP/ADP/AMP的ratio
最好能有ATP/ADP/AMP的ratio
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