f*t
2 楼
女人YY起来，古今中外都是一样。电影里是女主，微微一笑里是编剧。好可怕。
e*r
3 楼
试过restore，但是出现3194错误。是因为不开放SHSH的原因？
P*y
4 楼
Does anyone know currently whether it is feasible to do whole-genome
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
y*g
5 楼
哇。。。这部是悬疑经典电影。。。平行世界里的佛洛依德心理空间。。。
【在 f******t 的大作中提到】
: 女人YY起来，古今中外都是一样。电影里是女主，微微一笑里是编剧。好可怕。
【在 f******t 的大作中提到】
: 女人YY起来，古今中外都是一样。电影里是女主，微微一笑里是编剧。好可怕。
N*n
6 楼
some people did microarray analysis using LCM tissue. it could be doable.
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
P*y
7 楼
We have no problem to do microarray and RNA-seq with LCM tissue. There are
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
P*y
8 楼
Does anyone know currently whether it is feasible to do whole-genome
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
bisulphite sequencing using tissues from Laser capture microdissection (LCM)
? Are there any protocol available to amplify genome DNA from LCM tissue?
Thanks!
N*n
9 楼
some people did microarray analysis using LCM tissue. it could be doable.
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
@Luo Liqun Lab
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
P*y
10 楼
We have no problem to do microarray and RNA-seq with LCM tissue. There are
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
also a lot of reports regarding this. But couldn't find a literature or
protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 N******n 的大作中提到】
: some people did microarray analysis using LCM tissue. it could be doable.
: @Luo Liqun Lab
:
: LCM)
s*9
11 楼
DNA should be easier because you do not need to worry about RNA degradation
during the staining and capture process.
It is a reference for classic sequence of DNA sample using LCM tissues.
Good luck.
PMID:19934021
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
during the staining and capture process.
It is a reference for classic sequence of DNA sample using LCM tissues.
Good luck.
PMID:19934021
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
P*y
12 楼
Thank you.
However what I want is a protol that can amplify the whole genomic DNA from
LCM tissue, not several target genes. The point here is "whole-genome"
bisulphite sequencing.
degradation
【在 s****9 的大作中提到】
: DNA should be easier because you do not need to worry about RNA degradation
: during the staining and capture process.
: It is a reference for classic sequence of DNA sample using LCM tissues.
: Good luck.
: PMID:19934021
However what I want is a protol that can amplify the whole genomic DNA from
LCM tissue, not several target genes. The point here is "whole-genome"
bisulphite sequencing.
degradation
【在 s****9 的大作中提到】
: DNA should be easier because you do not need to worry about RNA degradation
: during the staining and capture process.
: It is a reference for classic sequence of DNA sample using LCM tissues.
: Good luck.
: PMID:19934021
b*n
13 楼
Why do you need to amplify genome DNA? QIAGEN just had a kit to make BS
library with 10ng of genome DNA.
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
library with 10ng of genome DNA.
LCM)
【在 P*********y 的大作中提到】
: Does anyone know currently whether it is feasible to do whole-genome
: bisulphite sequencing using tissues from Laser capture microdissection (LCM)
: ? Are there any protocol available to amplify genome DNA from LCM tissue?
: Thanks!
s*r
14 楼
能不能分享一下LCM tissue做microarray和RNA-seq的protocol?
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
【在 P*********y 的大作中提到】
: We have no problem to do microarray and RNA-seq with LCM tissue. There are
: also a lot of reports regarding this. But couldn't find a literature or
: protocol on whole-genome bisulphite sequencing using LCM tissues.
P*y
15 楼
There should be a number of published papers describing the protocol. You
can google it.
Here are a couple that I found:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156186/
http://dev.biologists.org/content/140/2/423.full
【在 s******r 的大作中提到】
: 能不能分享一下LCM tissue做microarray和RNA-seq的protocol?
can google it.
Here are a couple that I found:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2156186/
http://dev.biologists.org/content/140/2/423.full
【在 s******r 的大作中提到】
: 能不能分享一下LCM tissue做microarray和RNA-seq的protocol?
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