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双酶切后的粘末段# Biology - 生物学
a*0
1
Iphone6 plus 64GB screen sometimes flickling and unresponsive. 想拿到苹果店
trade in 成iPhone7。网上查可以拿$225 credit. 那些验收的人查的严吗? 如果运气
好,当时验收的时候不会闪屏,是不是就可以蒙混过关。 拿到$225 credit.
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l*1
2
因为要长期使用线性化后的载体,想着冻存后封装成小份使用。不知道双酶切载体后的
粘末端是否稳定?有同事说冻了两年以后还能用,不知道是不是这样?
多谢
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a*d
3
I have fragments stored at -20 for more than one year, they still work fine.
The concentration may decrease overtime though.
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j*n
4
my boss is still using his vectors made in 1980s
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q*7
5
Use this company's gel extraction kit will allow you get washing buffer-free
linearized vectors/DNA fragments, which will increase your cloning
efficiency.
You can store linearized DNA for over 5 years at -20C and still can ligate
to corresponding DNA fragment for over 90%.
http://www.greenbioresearch.com/gel-extraction-pcr-purification
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