What are biologists doing?# Biology - 生物学
x*n
1 楼
老人来了需要看中文电视，但是折腾各种链接实在太麻烦了，有没有已经解决了版权问
题的电视盒子？似乎乐视盒子没法购买（http://www.leeco.com/Chinese），有没有别的选择？
付费不要太离谱就行，谢谢！
题的电视盒子？似乎乐视盒子没法购买（http://www.leeco.com/Chinese），有没有别的选择？
付费不要太离谱就行，谢谢！
c*7
2 楼
如题
p*e
3 楼
请问有人给imac的hybird drive换过SSD吗？
我准备升级但是有一个问题
hybird drive是主板上一块容量很小但是速度快的ssd和一块普通hhd组成
升级后普通hhd被ssd换掉
那新的ssd和主板上面的快速ssd怎么协同工作？
格式化新硬盘和安装操作系统是不是有什么特殊的事情要做
我准备升级但是有一个问题
hybird drive是主板上一块容量很小但是速度快的ssd和一块普通hhd组成
升级后普通hhd被ssd换掉
那新的ssd和主板上面的快速ssd怎么协同工作？
格式化新硬盘和安装操作系统是不是有什么特殊的事情要做
s*r
4 楼
最近有需要进一个轻便的laptop. 喜欢14inch的. 看上了Lenovo的U430/430P系列. 好
像网上的选择不是太多,最近也没什么大的优惠活动. 在犹豫中. 有没有U430(p)的用户
来谈谈体验. 谢谢.
像网上的选择不是太多,最近也没什么大的优惠活动. 在犹豫中. 有没有U430(p)的用户
来谈谈体验. 谢谢.
n*m
5 楼
mmunology Retraction Exposes Misconduct
07/10/2013
Andrew S. Wiecek
Nature retracts a 1994 paper after the first author was found to have "most
likely" committed research misconduct. Learn more...
Last week, Nature retracted a highly-cited paper published in 1994 because
the authors were unable to reproduce the results, according to the
retraction notice (1). But the real reason behind the retraction might have
more to do with the first author’s scientific misconduct.
Nature has retracted a 1994 paper because first author Karel Bezouska was
found to have "most likely" committed research misconduct. Source: https://
www.natur.cuni.cz
In the 1994 paper (2), first author Karel Bezouska and colleagues from
Northwick Park Hospital in the UK, the Charles University of Prague, and the
Institute of Microbiology of the Czech Academy of Sciences reported that
they had identified carbohydrate oligosaccharide ligands that bound strongly
to NKR-P1, a membrane protein on natural killer cells. Natural killer cells
are part of the immune system. Their function is to identify and mark
virally infected cells and tumor cells for death.
Some tumor cells, however, are resistant to natural killer cells, allowing
them to evade targeted cell destruction. So when the researchers claimed in
their paper that incubating resistant tumor cells with the oligosaccharide
ligands they had identified made the cells vulnerable to elimination by
natural killer cells, it was considered a breakthrough.
“The findings were indeed considered a big at the time,” lead author Ten
Feizi, whose lab Bezouska was working in when he wrote the 1994 paper, said
in an email to BioTechniques. Feizi is now a professor at Imperial College
London.
Since publication, the paper has been cited 257 times since publication,
according to Thomson Reuters Web of Knowledge. “It is most likely that many
of the citations were by scientists unaware of the irreproducibility. I say
this because I made a point of informing key people in the field about the
problem, and between us when refereeing papers citing the article, we
discouraged citation,” said Feizi.
Two years later, a correction to the paper was published in Nature (3). In
that notice, the researchers said that they were unable to reproduce the
results. As a result, the team was re-evaluating the experimental system,
which was set up in Prague by three of the study authors: Bezouska, Anna
Fiserova, and Miloslav Pospíšil. The team promised to publish the
results of their re-evaluation as soon as possible.
But it wasn’t until just last week, 19 years after the original article was
published, that the paper was finally retracted. And that retraction came
without the signatures of Bezouska, Lawson, Pospíšil (now deceased),
and another author who could not be found.
As it turns out, the retraction only came to pass because first author
Bezouska was found by an ethics committee at Charles University in January
2013 to have “most likely” committed scientific misconduct in the
experiments published in the 1994 Nature paper and in follow-up studies, as
reported by Retraction Watch. As a result, Bezouska was terminated from the
Charles University and the Institute of Microbiology. In their press
releases, the 2 organizations urged his coauthors to review the more than
100 peer-reviewed publications that he had published for invalid results.
Although the irreproducible data from the 1994 paper were mentioned in the
report, the most conclusive evidence of Bezouska’s misconduct came from
Professor Vladimir Kren and three students from his laboratory at the
Institute of Microbiology who, in 2012, suspected someone was manipulating
their NKR-P1 carbohydrate binding experiments. So they installed a camera to
monitor their samples overnight. The camera caught Bezouska red-handed,
showing him manipulating the samples to generate positive results.
“…Bezouska fabricated practically all results dealing with NKR-P1 and CD69
receptors,” said Kren in an email to BioTechniques. CD69 receptors are
another surface protein on NK cells. Bezouska claimed to have identified
high-affinity ligands for CD69 receptors in a 1995 Biochemical and
Biophysical Research Communications paper (4). “At present, we estimate
that there [are] some 20 papers contaminated by this misconduct, and these
papers have to be corrected or retracted.”
Although Feizi wanted to retract the 1994 paper when the correction was
published in 1996, Nature’s editorial policy at the time required that
retractions be accompanied by the signature of all authors, according to an
email that she wrote to Retraction Watch. Feizi, however, did publish two
studies in other journals that re-evaluated the results from the 1994 Nature
paper and the 1995 Biochemical and Biophysical Research Communications
paper, concluding that all those results were irreproducible (5-6).
In addition, Feizi published a correction in 1999 to another paper that she
co-authored with Bezouska in 1994 in the Journal of Biological Chemistry,
also on the binding of carbohydrates to NKR-P1 (7-8).
References
1. Bezouska, K., C.-T. Yuen, J. O/'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fiserova, M. Pospisil, and T. Feizi. 2013. Retraction:
Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature advance online publication(July).
2. Bezouska, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M. Lawson
, K. Drbal, A. Fiserova, M. Posisil, and T. Feizi. 1994. Oligosaccharide
ligands for NKR-p1 protein activate NK cells and cytotoxicity. Nature 372(
6502):150-157.
3.Bezou[caron]ka, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fis[caron]erova, M. PospIs[caron]il, and T. Feizi. 1996
. Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature 380(6574):559.
4. Bezouska, K., A. Nepovím, O. Horváth, M. Pospísil, J. Hamann, and T.
Feizi. 1995. CD 69 antigen of human lymphocytes is a calcium-dependent
carbohydrate-binding protein. Biochemical and biophysical research
communications 208(1):68-74.
5. Kogelberg, H., A. M. Lawson, F. W. Muskett, R. A. Carruthers, and T.
Feizi. 2000. Expression in escherichia coli, folding in vitro, and
characterization of the carbohydrate recognition domain of the natural
killer cell receptor NKR-P1A. Protein Expression and Purification 20(1):10-
20.
6. Childs, R. A., C. Galustian, A. M. Lawson, G. Dougan, K. Benwell, G.
Frankel, and T. Feizi. 1999. Recombinant soluble human CD69 dimer produced
in escherichia coli: Reevaluation of saccharide binding. Biochemical and
Biophysical Research Communications 266(1):19-23.
7. Kogelberg, H., E. Montero, S. Bay, A. M. Lawson, and T. Feizi. 1999. Re-
evaluation of monosaccharide binding property of recombinant soluble
carbohydrate recognition domain of the natural killer cell receptor NKR-P1A.
Journal of Biological Chemistry 274(42):30335.
8. Bezouska, K., G. Vlahas, O. Horváth, G. Jinochová, A. Fiserová, R.
Giorda, W. H. Chambers, T. Feizi, and M. Pospísil. 1994. Rat natural killer
cell antigen, NKR-p1, related to c-type animal lectins is a carbohydrate-
binding protein. Journal of Biological Chemistry 269(24):16945-16952.
07/10/2013
Andrew S. Wiecek
Nature retracts a 1994 paper after the first author was found to have "most
likely" committed research misconduct. Learn more...
Last week, Nature retracted a highly-cited paper published in 1994 because
the authors were unable to reproduce the results, according to the
retraction notice (1). But the real reason behind the retraction might have
more to do with the first author’s scientific misconduct.
Nature has retracted a 1994 paper because first author Karel Bezouska was
found to have "most likely" committed research misconduct. Source: https://
www.natur.cuni.cz
In the 1994 paper (2), first author Karel Bezouska and colleagues from
Northwick Park Hospital in the UK, the Charles University of Prague, and the
Institute of Microbiology of the Czech Academy of Sciences reported that
they had identified carbohydrate oligosaccharide ligands that bound strongly
to NKR-P1, a membrane protein on natural killer cells. Natural killer cells
are part of the immune system. Their function is to identify and mark
virally infected cells and tumor cells for death.
Some tumor cells, however, are resistant to natural killer cells, allowing
them to evade targeted cell destruction. So when the researchers claimed in
their paper that incubating resistant tumor cells with the oligosaccharide
ligands they had identified made the cells vulnerable to elimination by
natural killer cells, it was considered a breakthrough.
“The findings were indeed considered a big at the time,” lead author Ten
Feizi, whose lab Bezouska was working in when he wrote the 1994 paper, said
in an email to BioTechniques. Feizi is now a professor at Imperial College
London.
Since publication, the paper has been cited 257 times since publication,
according to Thomson Reuters Web of Knowledge. “It is most likely that many
of the citations were by scientists unaware of the irreproducibility. I say
this because I made a point of informing key people in the field about the
problem, and between us when refereeing papers citing the article, we
discouraged citation,” said Feizi.
Two years later, a correction to the paper was published in Nature (3). In
that notice, the researchers said that they were unable to reproduce the
results. As a result, the team was re-evaluating the experimental system,
which was set up in Prague by three of the study authors: Bezouska, Anna
Fiserova, and Miloslav Pospíšil. The team promised to publish the
results of their re-evaluation as soon as possible.
But it wasn’t until just last week, 19 years after the original article was
published, that the paper was finally retracted. And that retraction came
without the signatures of Bezouska, Lawson, Pospíšil (now deceased),
and another author who could not be found.
As it turns out, the retraction only came to pass because first author
Bezouska was found by an ethics committee at Charles University in January
2013 to have “most likely” committed scientific misconduct in the
experiments published in the 1994 Nature paper and in follow-up studies, as
reported by Retraction Watch. As a result, Bezouska was terminated from the
Charles University and the Institute of Microbiology. In their press
releases, the 2 organizations urged his coauthors to review the more than
100 peer-reviewed publications that he had published for invalid results.
Although the irreproducible data from the 1994 paper were mentioned in the
report, the most conclusive evidence of Bezouska’s misconduct came from
Professor Vladimir Kren and three students from his laboratory at the
Institute of Microbiology who, in 2012, suspected someone was manipulating
their NKR-P1 carbohydrate binding experiments. So they installed a camera to
monitor their samples overnight. The camera caught Bezouska red-handed,
showing him manipulating the samples to generate positive results.
“…Bezouska fabricated practically all results dealing with NKR-P1 and CD69
receptors,” said Kren in an email to BioTechniques. CD69 receptors are
another surface protein on NK cells. Bezouska claimed to have identified
high-affinity ligands for CD69 receptors in a 1995 Biochemical and
Biophysical Research Communications paper (4). “At present, we estimate
that there [are] some 20 papers contaminated by this misconduct, and these
papers have to be corrected or retracted.”
Although Feizi wanted to retract the 1994 paper when the correction was
published in 1996, Nature’s editorial policy at the time required that
retractions be accompanied by the signature of all authors, according to an
email that she wrote to Retraction Watch. Feizi, however, did publish two
studies in other journals that re-evaluated the results from the 1994 Nature
paper and the 1995 Biochemical and Biophysical Research Communications
paper, concluding that all those results were irreproducible (5-6).
In addition, Feizi published a correction in 1999 to another paper that she
co-authored with Bezouska in 1994 in the Journal of Biological Chemistry,
also on the binding of carbohydrates to NKR-P1 (7-8).
References
1. Bezouska, K., C.-T. Yuen, J. O/'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fiserova, M. Pospisil, and T. Feizi. 2013. Retraction:
Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature advance online publication(July).
2. Bezouska, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M. Lawson
, K. Drbal, A. Fiserova, M. Posisil, and T. Feizi. 1994. Oligosaccharide
ligands for NKR-p1 protein activate NK cells and cytotoxicity. Nature 372(
6502):150-157.
3.Bezou[caron]ka, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fis[caron]erova, M. PospIs[caron]il, and T. Feizi. 1996
. Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature 380(6574):559.
4. Bezouska, K., A. Nepovím, O. Horváth, M. Pospísil, J. Hamann, and T.
Feizi. 1995. CD 69 antigen of human lymphocytes is a calcium-dependent
carbohydrate-binding protein. Biochemical and biophysical research
communications 208(1):68-74.
5. Kogelberg, H., A. M. Lawson, F. W. Muskett, R. A. Carruthers, and T.
Feizi. 2000. Expression in escherichia coli, folding in vitro, and
characterization of the carbohydrate recognition domain of the natural
killer cell receptor NKR-P1A. Protein Expression and Purification 20(1):10-
20.
6. Childs, R. A., C. Galustian, A. M. Lawson, G. Dougan, K. Benwell, G.
Frankel, and T. Feizi. 1999. Recombinant soluble human CD69 dimer produced
in escherichia coli: Reevaluation of saccharide binding. Biochemical and
Biophysical Research Communications 266(1):19-23.
7. Kogelberg, H., E. Montero, S. Bay, A. M. Lawson, and T. Feizi. 1999. Re-
evaluation of monosaccharide binding property of recombinant soluble
carbohydrate recognition domain of the natural killer cell receptor NKR-P1A.
Journal of Biological Chemistry 274(42):30335.
8. Bezouska, K., G. Vlahas, O. Horváth, G. Jinochová, A. Fiserová, R.
Giorda, W. H. Chambers, T. Feizi, and M. Pospísil. 1994. Rat natural killer
cell antigen, NKR-p1, related to c-type animal lectins is a carbohydrate-
binding protein. Journal of Biological Chemistry 269(24):16945-16952.
A*6
6 楼
买Roku或者Fire TV呗。 Roku与FireTV正式上线的中文电视应用，都是解决了版权与北
美服务器的问题。比如说Damai，Damai是由鹏博士集团在北美专门为华人朋友提供的中
文电视应用，有100多个直播电视台，上千部国内电影，电视剧，综艺，及少儿节目。
美服务器的问题。比如说Damai，Damai是由鹏博士集团在北美专门为华人朋友提供的中
文电视应用，有100多个直播电视台，上千部国内电影，电视剧，综艺，及少儿节目。
m*s
7 楼
DIY的后果就是你有50%的可能性烧了主板。
另外有25%的可能性烧了SSD
另外有25%的可能性烧了SSD
a*w
8 楼
为什么不考虑thinkpad t430s,比u430更轻。
g*j
9 楼
都是混饭呗
N*d
10 楼
用小米盒子，装几个app运行一下。就跟在国内一样，没有任何限制。直播现在也有。
想看剧，还可以语音搜索。
缺点是没有油管。不过现在的电视都有了。
想看剧，还可以语音搜索。
缺点是没有油管。不过现在的电视都有了。
T*s
11 楼
换它干啥 没用
s*r
12 楼
真没怎么注意t430s, 去看看.
l*t
13 楼
scientists are human too.
but I believe these are exceptions, not the norm.
【在 n*********m 的大作中提到】
: mmunology Retraction Exposes Misconduct
: 07/10/2013
: Andrew S. Wiecek
: Nature retracts a 1994 paper after the first author was found to have "most
: likely" committed research misconduct. Learn more...
: Last week, Nature retracted a highly-cited paper published in 1994 because
: the authors were unable to reproduce the results, according to the
: retraction notice (1). But the real reason behind the retraction might have
: more to do with the first author’s scientific misconduct.
: Nature has retracted a 1994 paper because first author Karel Bezouska was
but I believe these are exceptions, not the norm.
【在 n*********m 的大作中提到】
: mmunology Retraction Exposes Misconduct
: 07/10/2013
: Andrew S. Wiecek
: Nature retracts a 1994 paper after the first author was found to have "most
: likely" committed research misconduct. Learn more...
: Last week, Nature retracted a highly-cited paper published in 1994 because
: the authors were unable to reproduce the results, according to the
: retraction notice (1). But the real reason behind the retraction might have
: more to do with the first author’s scientific misconduct.
: Nature has retracted a 1994 paper because first author Karel Bezouska was
h*8
14 楼
麻烦问一下，买了小米盒子就可以看中文电视节目了？还用不用另外付费啊？谢谢
: 用小米盒子，装几个app运行一下。就跟在国内一样，没有任何限制。直播现在
也有。
: 想看剧，还可以语音搜索。
: 缺点是没有油管。不过现在的电视都有了。
【在 N**********d 的大作中提到】
: 用小米盒子，装几个app运行一下。就跟在国内一样，没有任何限制。直播现在也有。
: 想看剧，还可以语音搜索。
: 缺点是没有油管。不过现在的电视都有了。
: 用小米盒子，装几个app运行一下。就跟在国内一样，没有任何限制。直播现在
也有。
: 想看剧，还可以语音搜索。
: 缺点是没有油管。不过现在的电视都有了。
【在 N**********d 的大作中提到】
: 用小米盒子，装几个app运行一下。就跟在国内一样，没有任何限制。直播现在也有。
: 想看剧，还可以语音搜索。
: 缺点是没有油管。不过现在的电视都有了。
m*s
15 楼
硬盘太小了就不好装Windows OS了
【在 T*********s 的大作中提到】
: 换它干啥 没用
【在 T*********s 的大作中提到】
: 换它干啥 没用
p*n
16 楼
刚买一个u430p refurbished，刚开机就提示说找不到硬盘，正在返修中。
重量和做工都还不错，甚至有点儿macbook的意思，但感觉ideapad还是不如thinkpad质
量稳定
【在 s*********r 的大作中提到】
: 最近有需要进一个轻便的laptop. 喜欢14inch的. 看上了Lenovo的U430/430P系列. 好
: 像网上的选择不是太多,最近也没什么大的优惠活动. 在犹豫中. 有没有U430(p)的用户
: 来谈谈体验. 谢谢.
重量和做工都还不错，甚至有点儿macbook的意思，但感觉ideapad还是不如thinkpad质
量稳定
【在 s*********r 的大作中提到】
: 最近有需要进一个轻便的laptop. 喜欢14inch的. 看上了Lenovo的U430/430P系列. 好
: 像网上的选择不是太多,最近也没什么大的优惠活动. 在犹豫中. 有没有U430(p)的用户
: 来谈谈体验. 谢谢.
A*6
17 楼
小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使用的。
你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
i*l
18 楼
U系列的不要买，问题不少。google一下wifi问题解决了吗。
h*8
19 楼
Walmart 就有卖小米盒子的/mi box. 能看中文电视节目吗？
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使
用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
【在 A******6 的大作中提到】
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使
用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
【在 A******6 的大作中提到】
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
s*r
20 楼
这样啊. 本来我还是有点担心ideapad的问题, 我从没买过ideapad的机器. 看了下
T430s的, 想要人Hasewell的就放弃了. 同时就看了看T440s. 可惜我不fan thinkpad的
这个传统的样子
. 还有其他系列推荐码? 不限于Lenovo的.
T430s的, 想要人Hasewell的就放弃了. 同时就看了看T440s. 可惜我不fan thinkpad的
这个传统的样子
. 还有其他系列推荐码? 不限于Lenovo的.
A*6
21 楼
美版的Mi Box，看国内电视剧最好的app就是Youtube，但是看不了直播。看节目主要看
搜索，老人操作起来不方便。
【在 h*******8 的大作中提到】
: Walmart 就有卖小米盒子的/mi box. 能看中文电视节目吗？
:
:
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使
: 用的。
:
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
:
搜索，老人操作起来不方便。
【在 h*******8 的大作中提到】
: Walmart 就有卖小米盒子的/mi box. 能看中文电视节目吗？
:
:
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使
: 用的。
:
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
:
N*d
22 楼
我的是ebay上国内卖家小米3增强型。装了super root 和china tunnel(有兴趣自己到网
上搜一下),小米主页上除VIP节目没试过，其它的电影电视剧音乐都可以看。直播能不
能看跟国内相关机构间歇性抽风有关。我用HDP，比以前电视台少了，但央1，4啥的仍
然可以看。
【在 A******6 的大作中提到】
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
上搜一下),小米主页上除VIP节目没试过，其它的电影电视剧音乐都可以看。直播能不
能看跟国内相关机构间歇性抽风有关。我用HDP，比以前电视台少了，但央1，4啥的仍
然可以看。
【在 A******6 的大作中提到】
: 小米盒子国内版，不是美版的，在美国根本使用不了。下载的APP，也是国内使用的。
: 你可以看看论坛内，其它网友买了小米盒子，都反应使用不了。。。。
相关阅读
Science also start talking about POSTDOC problems!!!把千老在各个学校的福利列一下吧转bioinformatics施一公是要接清华校长的前奏啊到底什么是千老？诚聘生物和医学类兼职和全职朱镕基吴仪出卖药品食品审批权对产业破坏的深远影响生物学申请联帮政府技术员的工作的渠道有那些？谢谢诚聘生物和医学类兼职和全职Question about tumor study on mice小明，听说你大学专业是生物？意想不到的难得工作机会，可是我还没有毕业，怎么处理？谢谢诚聘生物和医学类兼职和全职哈佛医学院招postdoc真的需要4-6个月？普外科实现SCI论文零的突破关于肠道菌群宏基因组测序未来50年能搞定癌症吗？请教一个问题诚求审稿机会: RNA, protein structure, molecular dynamics谁给推荐个三四分的综合性杂志？