Protein phosphorylation - 未名空间MITBBS历史存档

M*M2013-07-31 07:07

1 楼

【以下文字转载自 NextGeneration 讨论区】
发信人: MommoM (MommoM), 信区: NextGeneration
标题: 宝宝在国内可以作为Dependent报税么
发信站: BBS 未名空间站 (Mon Feb 27 16:52:47 2012, 美东)
去年一年都在国内，现在可以作为dependent报税么？谢谢。

d*x2013-07-31 07:07

2 楼

I found my protein is phosphorylated and stabilized by phosphorylation,
which enhances its occupancy of target gene promoter, but decreases its
activity. Does anyone have any clue to explain this phenomenon?Thanks

d*g2013-07-31 07:07

3 楼

为什么不能呢？

d*x2013-07-31 07:07

4 楼

【在 d*x 的大作中提到】

: I found my protein is phosphorylated and stabilized by phosphorylation,
: which enhances its occupancy of target gene promoter, but decreases its
: activity. Does anyone have any clue to explain this phenomenon?Thanks

M*M2013-07-31 07:07

5 楼

不是说必须得与父母住满半年以上才能满足 residency test for qualifying child嘛？
不过也有temporary absence; vacation。所以不知道有人file过没有，没在身边一年
，还能不能file。

d*x2013-07-31 07:07

6 楼

conformation cahnge? any ideas

s*o2013-07-31 07:07

7 楼

是在美国出生的话,应该是可以的.

【在 M****M 的大作中提到】

: 不是说必须得与父母住满半年以上才能满足 residency test for qualifying child嘛？
: 不过也有temporary absence; vacation。所以不知道有人file过没有，没在身边一年
: ，还能不能file。

e*s2013-07-31 07:07

8 楼

Your information is too general. I believe that it is hard for anyone to
give any meaningful suggestions.
It is normal that phosphorylation changes protein's conformation.
I don't know much about protein-DNA interaction. But the enhancement of
protein's binding with target DNA sequence by phosphorylation should not be
a big surprise.
Regarding stability and activity of the protein, have no idea.
What made you think that the phosphorylation stabilizes the protein?

l*a2013-07-31 07:07

9 楼

这个不能把。
记得以前用保税软件，好像超过半年就不行了。跟在美国生的有关系吗？

【在 M****M 的大作中提到】

: 不是说必须得与父母住满半年以上才能满足 residency test for qualifying child嘛？
: 不过也有temporary absence; vacation。所以不知道有人file过没有，没在身边一年
: ，还能不能file。

d*x2013-07-31 07:07

10 楼

Thanks, Emases,
I did the half life of the protein, the phosphoralated protein more stable.

s*d2013-07-31 07:07

11 楼

有五条标准。

e*s2013-07-31 07:07

12 楼

Apparently, you have purified protein. Then why not trying to detect the
conformational change?
Limited proteolysis, fluorescence or other biophysical methods.

【在 d*x 的大作中提到】

: Thanks, Emases,
: I did the half life of the protein, the phosphoralated protein more stable.

l*a2013-07-31 07:07

13 楼

no

【在 M****M 的大作中提到】

: 不是说必须得与父母住满半年以上才能满足 residency test for qualifying child嘛？
: 不过也有temporary absence; vacation。所以不知道有人file过没有，没在身边一年
: ，还能不能file。

v*m2013-07-31 07:07

14 楼

activator conformation -----phosphorylated------> inhibitor conformation.

【在 d*x 的大作中提到】

: I found my protein is phosphorylated and stabilized by phosphorylation,
: which enhances its occupancy of target gene promoter, but decreases its
: activity. Does anyone have any clue to explain this phenomenon?Thanks

M*M2013-07-31 07:07

15 楼

难道大家都没有类似经验么

d*x2013-07-31 07:07

16 楼

I found my protein is phosphorylated and stabilized by phosphorylation,
which enhances its occupancy of target gene promoter, but decreases its
activity. Does anyone have any clue to explain this phenomenon?Thanks

s*l2013-07-31 07:07

17 楼

人家跟你说no了，你用turbo tax去试试就知道了，除非你撒谎。

【在 M****M 的大作中提到】

: 难道大家都没有类似经验么

d*x2013-07-31 07:07

18 楼

【在 d*x 的大作中提到】

: I found my protein is phosphorylated and stabilized by phosphorylation,
: which enhances its occupancy of target gene promoter, but decreases its
: activity. Does anyone have any clue to explain this phenomenon?Thanks

d*x2013-07-31 07:07

19 楼

conformation cahnge? any ideas

e*s2013-07-31 07:07

20 楼

Your information is too general. I believe that it is hard for anyone to
give any meaningful suggestions.
It is normal that phosphorylation changes protein's conformation.
I don't know much about protein-DNA interaction. But the enhancement of
protein's binding with target DNA sequence by phosphorylation should not be
a big surprise.
Regarding stability and activity of the protein, have no idea.
What made you think that the phosphorylation stabilizes the protein?

d*x2013-07-31 07:07

21 楼

Thanks, Emases,
I did the half life of the protein, the phosphoralated protein more stable.

e*s2013-07-31 07:07

22 楼

Apparently, you have purified protein. Then why not trying to detect the
conformational change?
Limited proteolysis, fluorescence or other biophysical methods.

【在 d*x 的大作中提到】

: Thanks, Emases,
: I did the half life of the protein, the phosphoralated protein more stable.

v*m2013-07-31 07:07

23 楼

activator conformation -----phosphorylated------> inhibitor conformation.

【在 d*x 的大作中提到】

: I found my protein is phosphorylated and stabilized by phosphorylation,
: which enhances its occupancy of target gene promoter, but decreases its
: activity. Does anyone have any clue to explain this phenomenon?Thanks

l*e2013-07-31 07:07

24 楼

DNA binding增加了多少？活性减少了多少？
从你提供的信息看，这个磷酸化有两个作用：
（1）磷酸化->稳定蛋白—>DNA binding增加，那么DNA binding增加就是由于蛋白多了
导致的（相当于overexpression）
（2）DNA binding 之后，转录活性降低，说明磷酸化抑制了transactivation
(1)和（2）不矛盾，结论应该是磷酸化的overall effect还是抑制转录活性