大家好,我现在正在做DNA damage的实验, 正准备rH2AX 的 ChIP-seq.
请问有无比较好的正对照? 就是在普通细胞里也会有rH2AX的结合位点. 看了相关的文
章,都没有找到很具体,可靠的位点.
文献:
1.
Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20796-801. doi: 10.1073/pnas.
1114677108. Epub 2011 Nov 21.
DNA double-strand breaks induced by high NaCl occur predominantly in gene
deserts.
Dmitrieva NI, Cui K, Kitchaev DA, Zhao K, Burg MB.
2.
Replication stress induces 53BP1-containing OPT
domains in G1 cells
Jeanine A. Harrigan,1 Rimma Belotserkovskaya,1 Julia Coates,1 Daniela S.
Dimitrova,2 Sophie E. Polo,1
Charles R. Bradshaw,1 Peter Fraser,2 and Stephen P. Jackson1
1The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England,
UK
2Laboratory of Chromatin and Gene Expression, Babraham Institute, Babraham
Research Campus, Cambridge CB22 3AT, England, UK
3.
Genome-wide profiles of H2AX and γ-H2AX differentiate endogenous and
exogenous DNA damage hotspots in human cells
Jungmin Seo1,
Sang Cheol Kim2,
Heun-Sik Lee1,
Jung Kyu Kim1,
Hye Jin Shon3,
Nur Lina Mohd Salleh4,
Kartiki Vasant Desai4,
Jae Ho Lee5,
Eun-Suk Kang3,
Jin Sung Kim6,* and
Jung Kyoon Choi4,7,*
