今天Nature这篇siDNA的文章大家有没有兴趣发散一下# Biology - 生物学
T*e
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http://www.nature.com/nature/journal/vaop/ncurrent/full/nature1
标题 DNA-guided DNA interference by a prokaryotic Argonaute
摘要
RNA interference is widely distributed in eukaryotes and has a variety of
functions, including antiviral defence and gene regulation1, 2. All RNA
interference pathways use small single-stranded RNA (ssRNA) molecules that
guide proteins of the Argonaute (Ago) family to complementary ssRNA targets:
RNA-guided RNA interference1, 2. The role of prokaryotic Ago variants has
remained elusive, although bioinformatics analysis has suggested their
involvement in host defence3. Here we demonstrate that Ago of the bacterium
Thermus thermophilus (TtAgo) acts as a barrier for the uptake and
propagation of foreign DNA. In vivo, TtAgo is loaded with 5′-phosphorylated
DNA guides, 13–25 nucleotides in length, that are mostly plasmid derived
and have a strong bias for a 5′-end deoxycytidine. These small interfering
DNAs guide TtAgo to cleave complementary DNA strands. Hence, despite
structural homology to its eukaryotic counterparts, TtAgo functions in host
defence by DNA-guided DNA interference.
大意
1. 转化得到多少plasmid的效果跟这个DNA介导的DNA干扰有关
用突变掉这个Ago酶的菌株，以后max prep都可以被mini prep替换了？
2. DNA作引子可以切割DNA
有点像CRISPR啊
3. 新的细菌免疫机制
标题 DNA-guided DNA interference by a prokaryotic Argonaute
摘要
RNA interference is widely distributed in eukaryotes and has a variety of
functions, including antiviral defence and gene regulation1, 2. All RNA
interference pathways use small single-stranded RNA (ssRNA) molecules that
guide proteins of the Argonaute (Ago) family to complementary ssRNA targets:
RNA-guided RNA interference1, 2. The role of prokaryotic Ago variants has
remained elusive, although bioinformatics analysis has suggested their
involvement in host defence3. Here we demonstrate that Ago of the bacterium
Thermus thermophilus (TtAgo) acts as a barrier for the uptake and
propagation of foreign DNA. In vivo, TtAgo is loaded with 5′-phosphorylated
DNA guides, 13–25 nucleotides in length, that are mostly plasmid derived
and have a strong bias for a 5′-end deoxycytidine. These small interfering
DNAs guide TtAgo to cleave complementary DNA strands. Hence, despite
structural homology to its eukaryotic counterparts, TtAgo functions in host
defence by DNA-guided DNA interference.
大意
1. 转化得到多少plasmid的效果跟这个DNA介导的DNA干扰有关
用突变掉这个Ago酶的菌株，以后max prep都可以被mini prep替换了？
2. DNA作引子可以切割DNA
有点像CRISPR啊
3. 新的细菌免疫机制
