Did anyone conjugate micelles with biomolecules? (转载)# Biology - 生物学
t*3
1 楼
下月换工作，两边都不支持EB1B,想试下eb1a，
但是不知道如果换到新工作再申请EB1A，是不是还能用现在的推荐信，因为现在推荐信
里都涉及了现在单位的信息
但是不知道如果换到新工作再申请EB1A，是不是还能用现在的推荐信，因为现在推荐信
里都涉及了现在单位的信息
n*g
2 楼
【 以下文字转载自 Chemistry 讨论区 】
发信人: nantsing (猛龙过江), 信区: Chemistry
标 题: Did anyone conjugate micelles with biomolecules?
发信站: BBS 未名空间站 (Sat May 3 01:44:19 2014, 美东)
Micelles were prepared by Phospholipid-PEG-COOH.EDC/NHS was used to activate
micelle surface to react with NH2-protein. However, never see the
conjugation is successful.
Very confused. Any suggestions?
发信人: nantsing (猛龙过江), 信区: Chemistry
标 题: Did anyone conjugate micelles with biomolecules?
发信站: BBS 未名空间站 (Sat May 3 01:44:19 2014, 美东)
Micelles were prepared by Phospholipid-PEG-COOH.EDC/NHS was used to activate
micelle surface to react with NH2-protein. However, never see the
conjugation is successful.
Very confused. Any suggestions?
t*3
3 楼
主要推荐信找的很费劲，其中5个独立推荐人，有俩三比较牛的人。实在不想浪费，但
是他们的推荐信确实都涉及到了现在工作的地点和工作
【在 t*****3 的大作中提到】
: 下月换工作，两边都不支持EB1B,想试下eb1a，
: 但是不知道如果换到新工作再申请EB1A，是不是还能用现在的推荐信，因为现在推荐信
: 里都涉及了现在单位的信息
是他们的推荐信确实都涉及到了现在工作的地点和工作
【在 t*****3 的大作中提到】
: 下月换工作，两边都不支持EB1B,想试下eb1a，
: 但是不知道如果换到新工作再申请EB1A，是不是还能用现在的推荐信，因为现在推荐信
: 里都涉及了现在单位的信息
A*y
4 楼
What's your reaction condition? You need to first make the activated NHS
ester PEG Phospholipid (or buy it if you can) in organic solvent. After
that you can do it in PBS buffer etc. If you run the EDC/NHS activation in
water solution, it won't work.
ester PEG Phospholipid (or buy it if you can) in organic solvent. After
that you can do it in PBS buffer etc. If you run the EDC/NHS activation in
water solution, it won't work.
t*3
5 楼
up下
n*g
6 楼
I run the EDC/NHS activation in water solution. Can you explain the reason
for not working? Thanks.
in
【在 A******y 的大作中提到】
: What's your reaction condition? You need to first make the activated NHS
: ester PEG Phospholipid (or buy it if you can) in organic solvent. After
: that you can do it in PBS buffer etc. If you run the EDC/NHS activation in
: water solution, it won't work.
for not working? Thanks.
in
【在 A******y 的大作中提到】
: What's your reaction condition? You need to first make the activated NHS
: ester PEG Phospholipid (or buy it if you can) in organic solvent. After
: that you can do it in PBS buffer etc. If you run the EDC/NHS activation in
: water solution, it won't work.
A*y
7 楼
Water, H2O, is a nucleophile. It is competing with your NHS during the
activation. You will get the acid back. EDC activation need to be without
water.
【在 n******g 的大作中提到】
: I run the EDC/NHS activation in water solution. Can you explain the reason
: for not working? Thanks.
:
: in
activation. You will get the acid back. EDC activation need to be without
water.
【在 n******g 的大作中提到】
: I run the EDC/NHS activation in water solution. Can you explain the reason
: for not working? Thanks.
:
: in
n*g
8 楼
I did follow Pierce instructions for COOH to amine-protein link in water
solution using EDC/NHS activation or just EDC activation.
Its protocol did work well for polymer microbead-COOH and amine-protein
linking. But it seems not working if I change microbead-COOH to micelle-COOH.
I do see people using maleimide-PEG-Phosphilipid to react with thiolated
protein.
without
【在 A******y 的大作中提到】
: Water, H2O, is a nucleophile. It is competing with your NHS during the
: activation. You will get the acid back. EDC activation need to be without
: water.
solution using EDC/NHS activation or just EDC activation.
Its protocol did work well for polymer microbead-COOH and amine-protein
linking. But it seems not working if I change microbead-COOH to micelle-COOH.
I do see people using maleimide-PEG-Phosphilipid to react with thiolated
protein.
without
【在 A******y 的大作中提到】
: Water, H2O, is a nucleophile. It is competing with your NHS during the
: activation. You will get the acid back. EDC activation need to be without
: water.
A*y
9 楼
Are you sure the one you buy is not already activated? EDC activation
require anhydrous condition (just go search online). Once Osu-ester is form
, it can react with amine in aqueous condition. If you think it is the
micelle, then you have to re-think you sequence of reaction.
COOH.
【在 n******g 的大作中提到】
: I did follow Pierce instructions for COOH to amine-protein link in water
: solution using EDC/NHS activation or just EDC activation.
: Its protocol did work well for polymer microbead-COOH and amine-protein
: linking. But it seems not working if I change microbead-COOH to micelle-COOH.
: I do see people using maleimide-PEG-Phosphilipid to react with thiolated
: protein.
:
: without
require anhydrous condition (just go search online). Once Osu-ester is form
, it can react with amine in aqueous condition. If you think it is the
micelle, then you have to re-think you sequence of reaction.
COOH.
【在 n******g 的大作中提到】
: I did follow Pierce instructions for COOH to amine-protein link in water
: solution using EDC/NHS activation or just EDC activation.
: Its protocol did work well for polymer microbead-COOH and amine-protein
: linking. But it seems not working if I change microbead-COOH to micelle-COOH.
: I do see people using maleimide-PEG-Phosphilipid to react with thiolated
: protein.
:
: without
n*g
10 楼
My understanding is that EDC activation can be done in water (pierce website
, please search EDC).
DSPE does have ammonium ion. Do you think ammonium ion cause the problem?
form
【在 A******y 的大作中提到】
: Are you sure the one you buy is not already activated? EDC activation
: require anhydrous condition (just go search online). Once Osu-ester is form
: , it can react with amine in aqueous condition. If you think it is the
: micelle, then you have to re-think you sequence of reaction.
:
: COOH.
, please search EDC).
DSPE does have ammonium ion. Do you think ammonium ion cause the problem?
form
【在 A******y 的大作中提到】
: Are you sure the one you buy is not already activated? EDC activation
: require anhydrous condition (just go search online). Once Osu-ester is form
: , it can react with amine in aqueous condition. If you think it is the
: micelle, then you have to re-think you sequence of reaction.
:
: COOH.
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