f*g
2 楼
想做个transgenic小鼠(over-express one gene in a mouse),感觉可能会致死,所以
想做inducible knockin mouse。
请问哪位知道些具体的,能说说吗?
谢谢
想做inducible knockin mouse。
请问哪位知道些具体的,能说说吗?
谢谢
h*2
3 楼
windows.old是用来保存以前的系统的。比如你从win 8升级到win 10, windows.old用
来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
你确信你再不会downgrade,可以把windows.old删掉:
To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
click the Start button, search for “Disk cleanup” in the Start menu, and
launch the Disk cleanup application. You can also right-click the C: drive
in File Explorer, select Properties, and click the “Disk Clean-up” button
under the General tab.
【在 a***e 的大作中提到】
: 尼玛个20G的folder,大拿们说说!
来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
你确信你再不会downgrade,可以把windows.old删掉:
To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
click the Start button, search for “Disk cleanup” in the Start menu, and
launch the Disk cleanup application. You can also right-click the C: drive
in File Explorer, select Properties, and click the “Disk Clean-up” button
under the General tab.
【在 a***e 的大作中提到】
: 尼玛个20G的folder,大拿们说说!
s*s
4 楼
1)inducible overexpression with mutant with rtTA/TO system
2)如果基因KO不lethal的话
LSL system with KI.
KI first, then add LSL 在前面。用Cre诱导表达。参见K-RasG12D LSL小鼠模型。
参见
http://blog.sina.com.cn/s/articlelist_5058859129_0_1.html
2)如果基因KO不lethal的话
LSL system with KI.
KI first, then add LSL 在前面。用Cre诱导表达。参见K-RasG12D LSL小鼠模型。
参见
http://blog.sina.com.cn/s/articlelist_5058859129_0_1.html
f*g
6 楼
谢谢了
【在 s*********s 的大作中提到】
: 1)inducible overexpression with mutant with rtTA/TO system
: 2)如果基因KO不lethal的话
: LSL system with KI.
: KI first, then add LSL 在前面。用Cre诱导表达。参见K-RasG12D LSL小鼠模型。
: 参见
: http://blog.sina.com.cn/s/articlelist_5058859129_0_1.html
【在 s*********s 的大作中提到】
: 1)inducible overexpression with mutant with rtTA/TO system
: 2)如果基因KO不lethal的话
: LSL system with KI.
: KI first, then add LSL 在前面。用Cre诱导表达。参见K-RasG12D LSL小鼠模型。
: 参见
: http://blog.sina.com.cn/s/articlelist_5058859129_0_1.html
a*e
9 楼
多谢
【在 h*****2 的大作中提到】
: windows.old是用来保存以前的系统的。比如你从win 8升级到win 10, windows.old用
: 来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
: 你确信你再不会downgrade,可以把windows.old删掉:
: To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
: click the Start button, search for “Disk cleanup” in the Start menu, and
: launch the Disk cleanup application. You can also right-click the C: drive
: in File Explorer, select Properties, and click the “Disk Clean-up” button
: under the General tab.
【在 h*****2 的大作中提到】
: windows.old是用来保存以前的系统的。比如你从win 8升级到win 10, windows.old用
: 来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
: 你确信你再不会downgrade,可以把windows.old删掉:
: To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
: click the Start button, search for “Disk cleanup” in the Start menu, and
: launch the Disk cleanup application. You can also right-click the C: drive
: in File Explorer, select Properties, and click the “Disk Clean-up” button
: under the General tab.
q*u
11 楼
rollback只保留一个月。
button
【在 h*****2 的大作中提到】
: windows.old是用来保存以前的系统的。比如你从win 8升级到win 10, windows.old用
: 来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
: 你确信你再不会downgrade,可以把windows.old删掉:
: To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
: click the Start button, search for “Disk cleanup” in the Start menu, and
: launch the Disk cleanup application. You can also right-click the C: drive
: in File Explorer, select Properties, and click the “Disk Clean-up” button
: under the General tab.
button
【在 h*****2 的大作中提到】
: windows.old是用来保存以前的系统的。比如你从win 8升级到win 10, windows.old用
: 来保存win 8的内容。如果有一天你又想downgrade到win 8,那么,就很简单了。如果
: 你确信你再不会downgrade,可以把windows.old删掉:
: To delete this folder, use the Windows Disk Cleanup tool. On Windows 10,
: click the Start button, search for “Disk cleanup” in the Start menu, and
: launch the Disk cleanup application. You can also right-click the C: drive
: in File Explorer, select Properties, and click the “Disk Clean-up” button
: under the General tab.
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