求问CMV promoter在ESCs胚胎干细胞系沉默问题？ - 未名空间MITBBS历史存档

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求问CMV promoter在ESCs胚胎干细胞系沉默问题？

求问CMV promoter在ESCs胚胎干细胞系沉默问题？# Biology - 生物学

s*e2014-07-10 07:07

1 楼

Approval and Denial Statistics for I-140, Immigrant Petition for Alien
Workers：
http://www.uscis.gov/portal/site/uscis/menuitem.5af9bb95919f35e

r*e2014-07-10 07:07

2 楼

还好，她是个女人

V*t2014-07-10 07:07

3 楼

CMV promoter 在mESCs里面会沉默，对吧，比如mE14、J1，用CMV驱动表达是不行的。
但是我想用minimal MCMV promoter，前面接一个multimerized DNA binding sites，
后面表达mCherry，在ES细胞表达能结合DNA的转录因子，看mCherry。这个会有问题吗
？我的载体将会是
-DNA binding sites-minimal MCMV promoter-mCherry-PGK-puro-
如果不行。pGL4载体是用minimal Promoter，驱动luciferase，想问这个minimal
Promoter是啥？能用在mE14吗？可以用GFP换掉后面的luciferase吗，有这样的载体可
以分享／买的吗？最后得到一个这样萌萌哒的载体：
- DNA binding sites - minimal Promoter - mCherry - SV40 early enhancer/
promoter- hygro -
这个用在mE4没问题吧？
谢谢！

l*t2014-07-10 07:07

4 楼

每年名额是多少啊？09年批了那么多

B*e2014-07-10 07:07

5 楼

这布很结实么，有木有！！尼玛谁还敢歧视材料学专业的！！！

V*t2014-07-10 07:07

6 楼

u*52014-07-10 07:07

7 楼

It would be better if USCIS provides approval rates of DIY and hire-a-lawyer
cases...
Then probably all immigration attorneys will lose their jobs...

c*g2014-07-10 07:07

8 楼

why? you think approval rate of cases handled by lawyers are much lower
than DIY?

lawyer

【在 u******5 的大作中提到】

: It would be better if USCIS provides approval rates of DIY and hire-a-lawyer
: cases...
: Then probably all immigration attorneys will lose their jobs...

b*r2014-07-10 07:07

9 楼

re

【在 c*******g 的大作中提到】

: why? you think approval rate of cases handled by lawyers are much lower
: than DIY?
:
: lawyer

l*t2014-07-10 07:07

10 楼

USCIS updated the table. In 2010, the approval rate for eb1a and eb1b were
62% and 91%.
http://www.uscis.gov/portal/site/uscis/menuitem.5af9bb95919f35e

d*22014-07-10 07:07

11 楼

not bad for 2010

【在 l******t 的大作中提到】

: USCIS updated the table. In 2010, the approval rate for eb1a and eb1b were
: 62% and 91%.
: http://www.uscis.gov/portal/site/uscis/menuitem.5af9bb95919f35e

d*i2014-07-10 07:07

12 楼

看了这个EB1B的统计，信心大增。准备推荐信去了！谢谢LZ。

r*n2014-07-10 07:07

13 楼

How do you it is for EB1B? I think it is for EB1A & B. Although the EB1B
passing rate may be higher.

【在 d*******i 的大作中提到】

: 看了这个EB1B的统计，信心大增。准备推荐信去了！谢谢LZ。

y*i2014-07-10 07:07

14 楼

see table title.

【在 r****n 的大作中提到】

: How do you it is for EB1B? I think it is for EB1A & B. Although the EB1B
: passing rate may be higher.

r*n2014-07-10 07:07

15 楼

Missed that. Thanks. Passing rate is high.

t*92014-07-10 07:07

16 楼

eb12 很淡定呀

【在 l******t 的大作中提到】

: USCIS updated the table. In 2010, the approval rate for eb1a and eb1b were
: 62% and 91%.
: http://www.uscis.gov/portal/site/uscis/menuitem.5af9bb95919f35e