After get extraction, why DNA quality is very bad? Can I still use it? - 未名空间MITBBS历史存档

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After get extraction, why DNA quality is very bad? Can I still use it?

After get extraction, why DNA quality is very bad? Can I still use it?# Biology - 生物学

f*w2014-09-04 07:09

1 楼

还有别的便宜方法吗，谢谢

h*g2014-09-04 07:09

2 楼

After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5,
260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to
make RNA. Thanks.

m*D2014-09-04 07:09

3 楼

Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D.
again.

h*g2014-09-04 07:09

4 楼

Yes, I eluted by ddH2O. So I should use TE buffer to elude? I will use it to
make RNA. Can I still use the DNA to make RNA? Thanks.

.

【在 m*********D 的大作中提到】

: Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D.
: again.

s*r2014-09-04 07:09

5 楼

probably not suitable for subsequent experiments. Is the DNA concentration
normal? One possibility is ethanol carryover.

x*e2014-09-04 07:09

6 楼

是有这个现象。做连接没问题，转录就不确定了。
我一般不用nanodrop测，直接跑胶看回收质量

,

【在 h*********g 的大作中提到】

: After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5,
: 260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to
: make RNA. Thanks.

m*D2014-09-04 07:09

7 楼

Yes,the DNA should be fine for transcription template. If you load on a
gel, you may even see ssDNA bands.
Better elude in TE. However if elude in ddH2O, you can add some NaCl
solution to 100 mM.this helps to form dsDNA.