e*p
2 楼
Pull down & Mass spec - a lot of proteins 60-80
Ask for help, - how to find the protein complex that my gene interacts with?
Ask for help, - how to find the protein complex that my gene interacts with?
A*e
4 楼
You need a database to exclude non-specific binding proteins. What epitope
did you tag on your protein? According to my experiences, more than 95%
proteins are not your interest. Your may try CRAPome online program to give
you a clue what are real hits.
did you tag on your protein? According to my experiences, more than 95%
proteins are not your interest. Your may try CRAPome online program to give
you a clue what are real hits.
e*p
6 楼
I used biotin-labeled - RNA pull down
thanks!
give
【在 A*****e 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: You need a database to exclude non-specific binding proteins. What epitope
: did you tag on your protein? According to my experiences, more than 95%
: proteins are not your interest. Your may try CRAPome online program to give
: you a clue what are real hits.
thanks!
give
【在 A*****e 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: You need a database to exclude non-specific binding proteins. What epitope
: did you tag on your protein? According to my experiences, more than 95%
: proteins are not your interest. Your may try CRAPome online program to give
: you a clue what are real hits.
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