检测荧光什么方法最sensitive# Biology - 生物学
m*p
1 楼
imaging方法除外
fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
来？(solution里面测)
fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
来？(solution里面测)
e*s
2 楼
相教于仪器，标记何种荧光分子对灵敏度的影响更大。
【在 m**p 的大作中提到】
: imaging方法除外
: fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
: 来？(solution里面测)
【在 m**p 的大作中提到】
: imaging方法除外
: fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
: 来？(solution里面测)
g*s
3 楼
我是用fluorometer
m*p
4 楼
同样的荧光标记和分子数量条件下呢？
【在 e****s 的大作中提到】
: 相教于仪器，标记何种荧光分子对灵敏度的影响更大。
【在 e****s 的大作中提到】
: 相教于仪器，标记何种荧光分子对灵敏度的影响更大。
A*y
5 楼
Luciferase is literally photon measurements. A lot of modern fluorometers (
i.e. plate readers) has the function to read luminescence. All you have to
do is look at their minimum detection range for the instruments. However, I
can guarantee you that more expensive ones will be better with exception of
instruments from molecular devices (old tech and still charge an arm and a
leg).
i.e. plate readers) has the function to read luminescence. All you have to
do is look at their minimum detection range for the instruments. However, I
can guarantee you that more expensive ones will be better with exception of
instruments from molecular devices (old tech and still charge an arm and a
leg).
c*0
6 楼
It depends on the optical instrumentation, illumination efficiency and
detector quantum efficiency. In other words, there is no most "sensitive"
one but the most suitable one according to budget limit and experiment
design.
detector quantum efficiency. In other words, there is no most "sensitive"
one but the most suitable one according to budget limit and experiment
design.
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