Bisulfite sequencing - 未名空间MITBBS历史存档

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Bisulfite sequencing

Bisulfite sequencing# Biology - 生物学

y*l2016-07-26 07:07

1 楼

National interest would be adversely affected if a Labor Certification were
required for the alien.
An alien seeking an exemption from the labor certification process must
present a national benefit so great as to outweigh the national interst
inherent in the labor certification process.
尤其是第一句话，到底啥意思啊？要了labor certification就对national interest不
利啦？所以不能要labor certificate？请大家指点。。。
优秀又不能要labor certificate才能NIW。优秀又可以拿labor certificate就只能
PERM，不能NIW？承认了很优秀，又说it has not be shown why labor
certification would be inappropriate in this case. 那要怎么SHOW才行呢？谢谢。

r*h2016-07-26 07:07

2 楼

各位前辈大神，
本人第一次做DNA甲基化检测，想检测药物处理后人类细胞某个特定启动子的甲基化情
况，可否指教一下到底该设计BSP引物还是MSP引物？测序用Sanger 法好还是
pyrosequencing？测序后的结果是用的哪个软件分析的呢？我看有些paper里面的图能
计算出某个位点的甲基化比例，也想那样分析。
Thanks in advance！
Ps: 我也很关注NgAgo，重复多次无法成功，期待韩老师早日出来指点迷经！

z*g2016-07-26 07:07

3 楼

co-ask. please advise...

S*e2016-07-26 07:07

4 楼

这个想顶一下，看过文章用pyro算比例，不知道靠不靠谱

【在 r*h 的大作中提到】

: 各位前辈大神，
: 本人第一次做DNA甲基化检测，想检测药物处理后人类细胞某个特定启动子的甲基化情
: 况，可否指教一下到底该设计BSP引物还是MSP引物？测序用Sanger 法好还是
: pyrosequencing？测序后的结果是用的哪个软件分析的呢？我看有些paper里面的图能
: 计算出某个位点的甲基化比例，也想那样分析。
: Thanks in advance！
: Ps: 我也很关注NgAgo，重复多次无法成功，期待韩老师早日出来指点迷经！

e*r2016-07-26 07:07

5 楼

The petitioner must persuasively demonstrate that the national interest
would be adversely affected if a labor certification were required for the
alien.
At least according to NYDOT, the protection of U.S. workers inherently
benefits the national interest. The national interest resulting from the
alien's contribution must exceed this inherent national interest. In other
words, an alien seeking a national interest waiver must prove that he or she
will serve the national interest to a substantially greater degree than
would an available U.S. worker having the same minimum qualifications. This
is a rather onerous requirement. The alien cannot simply play an important
role in a project which significantly benefits the field of endeavor; he or
she must personally present a significant benefit to the field of endeavor.

were
谢。

【在 y***l 的大作中提到】

: National interest would be adversely affected if a Labor Certification were
: required for the alien.
: An alien seeking an exemption from the labor certification process must
: present a national benefit so great as to outweigh the national interst
: inherent in the labor certification process.
: 尤其是第一句话，到底啥意思啊？要了labor certification就对national interest不
: 利啦？所以不能要labor certificate？请大家指点。。。
: 优秀又不能要labor certificate才能NIW。优秀又可以拿labor certificate就只能
: PERM，不能NIW？承认了很优秀，又说it has not be shown why labor
: certification would be inappropriate in this case. 那要怎么SHOW才行呢？谢谢。