Cas9-Crispr in mice# Biology - 生物学
r*1
1 楼
各位前辈：
本人转行用老鼠做实验， 现在要做Cas9-Crispr genome editing (in Mice). 想请有
经验的前辈推荐比较实用的书， 经典的paper and protocol.
谢谢！
本人转行用老鼠做实验， 现在要做Cas9-Crispr genome editing (in Mice). 想请有
经验的前辈推荐比较实用的书， 经典的paper and protocol.
谢谢！
z*5
2 楼
https://www.jove.com/video/55765/generation-genetically-modified-mice-
through-microinjection
through-microinjection
L*n
3 楼
© 2017
Genome Editing in Animals
Methods and Protocols
Editors: Hatada, Izuho (Ed.)
Includes cutting-edge methods and protocols
Provides step-by-step detail for reproducible results
Contains key notes and implementation advice from the experts
About this book
This volume details protocols that can be used for generation of knockout
animals. Chapters guide the reader through basic protocols for three genome
editing technologies, target design tools, and specific protocols for each
animal. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known
pitfalls.
Authoritative and practical, Genome Editing in Animals: Methods and
Protocols aims to ensure successful results in the further study of this
vital field.
Table of contents (20 chapters)
Construction and Evaluation of Zinc Finger Nucleases
Ochiai, Hiroshi (et al.)
Pages 1-24
Preview Buy Chapter $49.95
Current Overview of TALEN Construction Systems
Sakuma, Tetsushi (et al.)
Pages 25-36
CRISPR/Cas9
Hatada, Izuho (et al.)
Pages 37-42
Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-
Target Risks in Human and Mouse
Wang, Qingbo (et al.)
Pages 43-53
Genome Editing of Mouse by Cytoplasmic Injection
Horii, Takuro (et al.)
Pages 55-66
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing
SgRNA/Cas9 Expressing Plasmids
Noda, Taichi (et al.)
Pages 67-80
Genome Editing in Mouse and Rat by Electroporation
Kaneko, Takehito
Pages 81-89
Generation of Knock-in Mouse by Genome Editing
Fujii, Wataru
Pages 91-100
Genome Editing of Rat
Kaneko, Takehito
Pages 101-108
Gene Targeting in Rabbits: Single-Step Generation of Knock-out Rabbits by
Microinjection of CRISPR/Cas9 Plasmids
Kawano, Yoshihiro (et al.)
Pages 109-120
Genome Editing of Pig
Watanabe, Masahito (et al.)
Pages 121-139
Genome Editing of Monkey
Liu, Zhen (et al.)
Pages 141-152
Genome Editing Mediated by Primordial Germ Cell in Chicken
Han, Jae Yong (et al.)
Pages 153-163
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in
Zebrafish
Kawahara, Atsuo
Pages 165-173
Genome Editing of Medaka
Ansai, Satoshi (et al.)
Pages 175-188
A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis
Founders Using the CRISPR-Cas System
Sakane, Yuto (et al.)
Pages 189-203
Genome Editing of Silkworms
Tsubota, Takuya (et al.)
Pages 205-218
Genome Editing in the Cricket, Gryllus bimaculatus
Watanabe, Takahito (et al.)
Pages 219-233
Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Sasakura, Yasunori (et al.)
Pages 235-245
Genome Editing of C. elegans
Sugi, Takuma
Genome Editing in Animals
Methods and Protocols
Editors: Hatada, Izuho (Ed.)
Includes cutting-edge methods and protocols
Provides step-by-step detail for reproducible results
Contains key notes and implementation advice from the experts
About this book
This volume details protocols that can be used for generation of knockout
animals. Chapters guide the reader through basic protocols for three genome
editing technologies, target design tools, and specific protocols for each
animal. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known
pitfalls.
Authoritative and practical, Genome Editing in Animals: Methods and
Protocols aims to ensure successful results in the further study of this
vital field.
Table of contents (20 chapters)
Construction and Evaluation of Zinc Finger Nucleases
Ochiai, Hiroshi (et al.)
Pages 1-24
Preview Buy Chapter $49.95
Current Overview of TALEN Construction Systems
Sakuma, Tetsushi (et al.)
Pages 25-36
CRISPR/Cas9
Hatada, Izuho (et al.)
Pages 37-42
Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-
Target Risks in Human and Mouse
Wang, Qingbo (et al.)
Pages 43-53
Genome Editing of Mouse by Cytoplasmic Injection
Horii, Takuro (et al.)
Pages 55-66
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing
SgRNA/Cas9 Expressing Plasmids
Noda, Taichi (et al.)
Pages 67-80
Genome Editing in Mouse and Rat by Electroporation
Kaneko, Takehito
Pages 81-89
Generation of Knock-in Mouse by Genome Editing
Fujii, Wataru
Pages 91-100
Genome Editing of Rat
Kaneko, Takehito
Pages 101-108
Gene Targeting in Rabbits: Single-Step Generation of Knock-out Rabbits by
Microinjection of CRISPR/Cas9 Plasmids
Kawano, Yoshihiro (et al.)
Pages 109-120
Genome Editing of Pig
Watanabe, Masahito (et al.)
Pages 121-139
Genome Editing of Monkey
Liu, Zhen (et al.)
Pages 141-152
Genome Editing Mediated by Primordial Germ Cell in Chicken
Han, Jae Yong (et al.)
Pages 153-163
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in
Zebrafish
Kawahara, Atsuo
Pages 165-173
Genome Editing of Medaka
Ansai, Satoshi (et al.)
Pages 175-188
A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis
Founders Using the CRISPR-Cas System
Sakane, Yuto (et al.)
Pages 189-203
Genome Editing of Silkworms
Tsubota, Takuya (et al.)
Pages 205-218
Genome Editing in the Cricket, Gryllus bimaculatus
Watanabe, Takahito (et al.)
Pages 219-233
Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Sasakura, Yasunori (et al.)
Pages 235-245
Genome Editing of C. elegans
Sugi, Takuma
r*1
4 楼
多谢两位前辈指教， very helpful
相关阅读
问题：c-myc 的promotor 调控怎样冻存酵母strain求助：MSCV载体最大可以deliver多大的片段关于审稿人的资格问题，谢谢各位前辈了Randy Schekman 抵制 NSC找工作，学校的“Research Scientist“，该不该跳？question for bioinfo guruNormal Cell讨教大牛，关于RNAseq数据发表请教stromal vascular fraction （SVF) from white adipose tissue 的几个问题拜托英文好，经验丰富的老大解读！被拒了？唉，生活怎么这么难？ 找工作找得好沮丧！zzYeast问题求教J1签证怎么才能快速转到公司的H1b？Immunotherapy from U Penn有人接到过Journal of Immunology and Clinical Research这个杂志的邀请吗？技术问题: cMyc直接激活的基因有啥？E. Coli 原核表达酶，现在有什么公司质粒好用的，纯化的用什么方式好一点呢？ ni柱吗？求推荐一个repeating pipette有human cardiomyopathic heart的cDNA卖吗？