[合集] How to increase washing stringency in IP?# Biology - 生物学
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chick (Por Los Momentos Dif Ciles) 于 (Wed Jan 3 13:21:18 2007) 提到:
I'm working on the project involved something similar to Ch-IP. Recently, I
got very weird result, in some group or controls, the Non-fixation IP has
even stronger singal than the formaldehyde fixation. This could partialy be
explained by the over-crosslinking lowered the possible detecable targets.
But, obviously, I need more stringent washing condition, at least to
diminish the non-
chick (Por Los Momentos Dif Ciles) 于 (Wed Jan 3 13:21:18 2007) 提到:
I'm working on the project involved something similar to Ch-IP. Recently, I
got very weird result, in some group or controls, the Non-fixation IP has
even stronger singal than the formaldehyde fixation. This could partialy be
explained by the over-crosslinking lowered the possible detecable targets.
But, obviously, I need more stringent washing condition, at least to
diminish the non-