M*C
2 楼
同一个DNA样品，根据vector map，T3, T7 两个方向测序，primer都是测序中心提供。
T3的结果正常，T7却NO band, NO data。 再测一次，还是这样。
大家觉得这是怎么回事？thx
T3的结果正常，T7却NO band, NO data。 再测一次，还是这样。
大家觉得这是怎么回事？thx
A*0
3 楼
所以大家讨论的都是建行atm取钱不收费
【在 c******i 的大作中提到】
: 刚给父母寄的卡，没有取钱，只是在建行的atm上查询了一下看看使用效果如何，怎么
: 就被收费了？
【在 c******i 的大作中提到】
: 刚给父母寄的卡，没有取钱，只是在建行的atm上查询了一下看看使用效果如何，怎么
: 就被收费了？
b*8
4 楼
From Promega:
Due to a sequence
difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
Promoter Primer cannot be used for sequencing these expression vectors. The
sequence difference is downstream of the transcriptional start site and is
located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
specifically to prime sequencing reactions from the mammalian expression
vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
pALTER®-MAX (Cat.# Q5761).
Due to a sequence
difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
Promoter Primer cannot be used for sequencing these expression vectors. The
sequence difference is downstream of the transcriptional start site and is
located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
specifically to prime sequencing reactions from the mammalian expression
vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
pALTER®-MAX (Cat.# Q5761).
c*i
5 楼
求详情介绍. 原始链接
M*C
6 楼
原来如此！
多谢
The
【在 b***8 的大作中提到】
: From Promega:
: Due to a sequence
: difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
: polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
: Promoter Primer cannot be used for sequencing these expression vectors. The
: sequence difference is downstream of the transcriptional start site and is
: located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
: Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
: specifically to prime sequencing reactions from the mammalian expression
: vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
多谢
The
【在 b***8 的大作中提到】
: From Promega:
: Due to a sequence
: difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
: polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
: Promoter Primer cannot be used for sequencing these expression vectors. The
: sequence difference is downstream of the transcriptional start site and is
: located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
: Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
: specifically to prime sequencing reactions from the mammalian expression
: vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
相关阅读
华中农业大学正在招人paper help!microarray 分析， 差异表达基因很多，但没有指向性怎么办？欢迎对医疗影像有兴趣的朋友加盟Paper help 包子酬谢招Guest Editor，cancer方向细胞长的很慢 是否是支原体污染？ 大神们救命。。。zz美国归侨企业家许晓椿：一个懂技术的商人搞不懂学生物学的脑子里都在想什么？大家还记得浙大的涂博士吗？接受博后offer时工资的问题co-first 第一位 vs co-first第二位 +co responding求一个pmCherry-1载体paper helpQuestions about ADCC assay with human PBMCs, thank you!走生物创业的一个好例子job opportunity (cell therapy) (转载)paper help please!! 发bao zi!1岁的公老鼠了，怎么让他使萝利母鼠怀孕？请问点学术问题。