Re: What method do you use for DNA transfection?# Biology - 生物学
R*s
1 楼
yes, immortalized cell lines are generally harder to transfect
than primary cells.
however, 283 in general should be easy to transfect unless your
genes are really toxic to the cells (some of genes I have to use
are in such case).
Some guy upstair mentioned Superfect Reagent from Qiagen.
That one is quite effective, and better than LipofectAmine
in many cases, but more expensive too.
than primary cells.
however, 283 in general should be easy to transfect unless your
genes are really toxic to the cells (some of genes I have to use
are in such case).
Some guy upstair mentioned Superfect Reagent from Qiagen.
That one is quite effective, and better than LipofectAmine
in many cases, but more expensive too.