Re: SOS! 请教如何防止蛋白质在sample buffer 中沉淀# Biology - 生物学
r*n
1 楼
Good point!
I am sure that the precipition was caused by potassium ions which complex with
SDS and become insoluble. You should remove potassium ions from your SDS
extraction solution by using sodium salt.
I do think your protein concentration is too high because I even used much
higher protein concentration for my protein 2d electrophoresis. But for
SDS-PAGE, you can not load too much protein even with a concentrated sample.
You still have to dilute your sample. I usually load 20-30 ug for my
I am sure that the precipition was caused by potassium ions which complex with
SDS and become insoluble. You should remove potassium ions from your SDS
extraction solution by using sodium salt.
I do think your protein concentration is too high because I even used much
higher protein concentration for my protein 2d electrophoresis. But for
SDS-PAGE, you can not load too much protein even with a concentrated sample.
You still have to dilute your sample. I usually load 20-30 ug for my