Re: 免疫沉降--寻求帮助# Biology - 生物学
s*y
1 楼
Just like other gus have already said:
1. Buy another antibody from another specie. (I highly recommend this way)
2. crosslink your antibody to gel particles (CNBr activated Sepharose),
use the gel to pull down your protein,then use high salt(eg., 8M urea) to
seperate your protein.
3. crosslink your antibody to your protein by EDC-NHS method, then your
target will have a higher molecular weight. However, the result can turn out
to be hard to interpreted since the antibody would also bind somethi
1. Buy another antibody from another specie. (I highly recommend this way)
2. crosslink your antibody to gel particles (CNBr activated Sepharose),
use the gel to pull down your protein,then use high salt(eg., 8M urea) to
seperate your protein.
3. crosslink your antibody to your protein by EDC-NHS method, then your
target will have a higher molecular weight. However, the result can turn out
to be hard to interpreted since the antibody would also bind somethi